Uptake of Fe-fraxetin complexes, an IRT1 independent strategy for iron acquisition in Arabidopsis thaliana
Uptake of Fe-fraxetin complexes, an IRT1 independent strategy for iron acquisition in Arabidopsis thaliana
复制标题
Fe-fraxetin 复合物的摄取，拟南芥铁获取的 IRT1 独立策略
DOI：
10.1101/2021.08.03.454955
复制
发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Esther Izquierdo
中科院分区：
文献类型：
--
作者：
Kevin Robe;Max Stassen;Joseph Chamieh;Philippe Gonzalez;S. Hem;V. Santoni;C. Dubos;Esther Izquierdo
铁是植物生长发育所必需的微量营养素。对大多数植物来说，在碱性土壤中吸收铁是一个挑战。在本研究中，我们研究了儿茶酚类香豆素黄酮类和黄酮类在植物铁获取中的作用及其铁螯合特性。质谱法和毛细管电泳法对铁-香豆素配合物进行了表征。为了了解这些复合物的作用，遗传学、分子和生物化学的方法被部署。我们证明了儿茶酚香豆素通过ATP依赖机制被拟南芥的根部吸收，并且在IRT1（主要的高亲和力铁输入源）或bHLH121（铁缺乏反应的关键调节因子）缺陷的植物可以通过外源供应的黄曲素和较小程度的黄曲素来补充。我们还发现铁和黄曲霉素可以在中性到碱性的pH值下形成稳定的配合物，可以被植物吸收。综上所述，这些结果表明，在高pH条件下，黄曲霉素可以通过直接将铁（III）转运到根部，绕过FRO2/IRT1系统，以类似于草中的植物铁载体的方式改善铁营养。这种策略可以解释非草类物种如何在碱性土壤中茁壮成长。
Iron (Fe) is a micronutrient essential for plant growth and development. Iron uptake in alkaline soil is a challenge for most plants. In this study, we investigated the role of the catechol coumarins fraxetin and esculetin in plant Fe acquisition and their Fe chelating properties. Mass spectrometry and capillary electrophoresis were used to characterize Fe-coumarin complexes. To understand the role of these complexes, genetic, molecular and biochemical approaches were deployed. We demonstrated that catechol coumarins are taken up by Arabidopsis thaliana root via an ATP dependent mechanism and that plants defective in IRT1 activity (the main high affinity Fe importer) or bHLH121 (a key regulator of Fe deficiency responses) can be complemented by exogenous supply of fraxetin and to a lesser extent of esculetin. We also showed that Fe and fraxetin can form stable complexes at neutral to alkaline pH that can be taken up by the plant. Overall, these results indicate that at high pH, fraxetin can improve Fe nutrition by directly transporting Fe(III) into the root, circumventing the FRO2/IRT1 system, in a similar way as phytosiderophores do in grasses. This strategy may explain how non-grass species can thrive in alkaline soils.
影响因子：
14.8
作者：
Rajniak J;Giehl RFH;Chang E;Murgia I;von Wirén N;Sattely ES
通讯作者：
Sattely ES
影响因子：
3.5
作者：
Baune, Matthias;Kang, Kyounglim;Weber, Guenther
通讯作者：
Weber, Guenther