Cellular signaling circuits interfaced with synthetic, post-translational, negating Boolean logic devices.
Cellular signaling circuits interfaced with synthetic, post-translational, negating Boolean logic devices.
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DOI：
10.1021/sb500222z
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发表时间：
2014-09-19
影响因子：
4.7
通讯作者：
Inoue, Takanari
中科院分区：
文献类型：
--
作者：
Razavi, Shiva;Su, Steven;Inoue, Takanari
关键词：
否定功能是细胞和计算机内部逻辑电路信息处理的基础。为了使未利用的电子概念适应细胞信号电路的询问，我们首先采取了自下而上的策略，即我们创建了基于蛋白质的设备，执行否定布尔逻辑运算，如NOT， NOR， NAND和N-IMPLY。这些装置在活细胞中通过精确地命令激活分子在三个亚细胞空间中的定位，在一分钟内起作用。我们将这些合成门连接到一个内源性信号回路，并设计了一个生理输出。为了寻找信号转导中的逻辑功能，我们接下来采取了自上而下的方法，并计算筛选了108个信号通路，以确定这些生物通路和电子电路之间的共同点和差异。这种综合和系统方法的结合将指导我们为复杂细胞信号的解构以及生物分子计算机的构建奠定基础。
A negating functionality is fundamental to information processing of logic circuits within cells and computers. Aiming to adapt unutilized electronic concepts to the interrogation of signaling circuits in cells, we first took a bottom-up strategy whereby we created protein-based devices that perform negating Boolean logic operations such as NOT, NOR, NAND, and N-IMPLY. These devices function in living cells within a minute by precisely commanding the localization of an activator molecule among three subcellular spaces. We networked these synthetic gates to an endogenous signaling circuit and devised a physiological output. In search of logic functions in signal transduction, we next took a top–down approach and computationally screened 108 signaling pathways to identify commonalities and differences between these biological pathways and electronic circuits. This combination of synthetic and systems approaches will guide us in developing foundations for deconstruction of intricate cell signaling, as well as construction of biomolecular computers.
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影响因子：
16.6
作者：
Lin, Yu-Chun;Nihongaki, Yuta;Inoue, Takanari
通讯作者：
Inoue, Takanari
影响因子：
56.9
作者：
Yeung, Tony;Gilbert, Gary E.;Grinstein, Sergio
通讯作者：
Grinstein, Sergio
影响因子：
21.3
作者：
Lambert, JM;Lambert, QT;Der, CJ
通讯作者：
Der, CJ
DOI：
10.1098/rsif.2011.0343
发表时间：
2012-03-07
期刊：
Journal of the Royal Society, Interface
影响因子：
--
作者：
Lakin MR;Youssef S;Cardelli L;Phillips A
通讯作者：
Phillips A
影响因子：
4.7
作者：
Miyamoto, Takafumi;Razavi, Shiva;DeRose, Robert;Inoue, Takanari
通讯作者：
Inoue, Takanari