In vivo CHI3L1 (YKL-40) expression in astrocytes in acute and chronic neurological diseases.
In vivo CHI3L1 (YKL-40) expression in astrocytes in acute and chronic neurological diseases.
复制标题
DOI：
10.1186/1742-2094-7-34
复制
发表时间：
2010-06-11
影响因子：
9.3
通讯作者：
Wiley CA
中科院分区：
文献类型：
--
作者：
Bonneh-Barkay D;Wang G;Starkey A;Hamilton RL;Wiley CA
CHI3L1(YKL-40)在各种炎症条件和癌症中表达上调。我们以前曾报道过慢病毒脑炎患者和非人类灵长类动物脑脊液中CHI3L1浓度升高，免疫组织化学结果显示CHI3L1与星形胶质细胞有关。在目前的研究中，我们评估了CHI3L1在多种急、慢性人类神经系统疾病中的转录和表达。酶联免疫吸附试验显示多发性硬化(MS)患者脑脊液中CHI3L1水平显著升高，且随年龄增长略有升高。原位杂交显示CHI3L1的转录主要与反应性星形胶质细胞有关，在慢性病毒性脑炎和MS等炎症状态下更为明显。比较CHI3L1在脑梗塞不同阶段的表达情况，发现YKL40在急性期在星形胶质细胞中大量表达，在慢性脑梗塞中表达减弱。综上所述，这些发现表明，CHI3L1在各种神经系统疾病的星形胶质细胞中被诱导，但它与炎性细胞区域的星形胶质细胞最为相关。
CHI3L1 (YKL-40) is up-regulated in a variety of inflammatory conditions and cancers. We have previously reported elevated CHI3L1 concentration in the cerebrospinal fluid (CSF) of human and non-human primates with lentiviral encephalitis and using immunohistochemistry showed that CHI3L1 was associated with astrocytes. In the current study CHI3L1 transcription and expression were evaluated in a variety of acute and chronic human neurological diseases. ELISA revealed significant elevation of CHI3L1 in the CSF of multiple sclerosis (MS) patients as well as mild elevation with aging. In situ hybridization (ISH) showed CHI3L1 transcription mostly associated with reactive astrocytes, that was more pronounced in inflammatory conditions like lentiviral encephalitis and MS. Comparison of CHI3L1 expression in different stages of brain infarction showed that YKL40 was abundantly expressed in astrocytes during acute phases and diminished to low levels in chronic infarcts. Taken together, these findings demonstrate that CHI3L1 is induced in astrocytes in a variety of neurological diseases but that it is most abundantly associated with astrocytes in regions of inflammatory cells.
登录
查看更多内容
影响因子：
11.5
作者：
Hormigo, Adilia;Gu, Bin;Holland, Eric C.
通讯作者：
Holland, Eric C.
影响因子：
2.3
作者：
Chung, C;Tallerico, T;Seeman, P
通讯作者：
Seeman, P
DOI：
10.1002/ajmg.b.30847
发表时间：
2009-06-05
影响因子：
2.8
作者：
Yamada, Kazuo;Hattori, Eiji;Yoshikawa, Takeo
通讯作者：
Yoshikawa, Takeo
影响因子：
9.3
作者：
Colton CA;Mott RT;Sharpe H;Xu Q;Van Nostrand WE;Vitek MP
通讯作者：
Vitek MP
DOI：
10.1084/jem.20081271
发表时间：
2009-05-11
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Lee CG;Hartl D;Lee GR;Koller B;Matsuura H;Da Silva CA;Sohn MH;Cohn L;Homer RJ;Kozhich AA;Humbles A;Kearley J;Coyle A;Chupp G;Reed J;Flavell RA;Elias JA
通讯作者：
Elias JA