Real-Time Whole-Genome Sequencing for Surveillance of Listeria monocytogenes, France.
Real-Time Whole-Genome Sequencing for Surveillance of Listeria monocytogenes, France.
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实时全基因组测序，用于法国单核细胞增生李斯特菌的监视。
DOI：
10.3201/eid2309.170336
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发表时间：
2017-09
影响因子：
11.8
通讯作者：
Lecuit M
中科院分区：
文献类型：
--
作者：
Moura A;Tourdjman M;Leclercq A;Hamelin E;Laurent E;Fredriksen N;Van Cauteren D;Bracq-Dieye H;Thouvenot P;Vales G;Tessaud-Rita N;Maury MM;Alexandru A;Criscuolo A;Quevillon E;Donguy MP;Enouf V;de Valk H;Brisse S;Lecuit M
在2015-2016年间，我们评估了全基因组测序(WGS)作为常规分型工具的性能。将其在李斯特菌病微生物和流行病学监测中的附加值与现行标准方法脉冲场凝胶电泳法(PFGE)进行比较。作为常规监测的一部分，共收集了2,743株单核细胞增多性李斯特菌，并对其进行了PFGE和从WGS提取的核心基因组多位点序列分型(CgMLST)。我们研究了包含人分离株的PFGE和cgMLST簇。CgMLST对菌株的识别率显著高于PFGE(p<；0.001)。CgMLST区分了具有相同PFGE图谱的无关菌株和具有不同PFGE图谱的亲缘关系密切的菌株。这一程序还改进了流行病学调查，仅包括系统发育密切相关的分离株，改进了来源识别，并促进了流行病学调查，使在早期阶段能够识别更多的疫情。WGS分型应取代PFGE成为单核细胞增多性李斯特菌的主要分型方法。
During 2015–2016, we evaluated the performance of whole-genome sequencing (WGS) as a routine typing tool. Its added value for microbiological and epidemiologic surveillance of listeriosis was compared with that for pulsed-field gel electrophoresis (PFGE), the current standard method. A total of 2,743 Listeria monocytogenes isolates collected as part of routine surveillance were characterized in parallel by PFGE and core genome multilocus sequence typing (cgMLST) extracted from WGS. We investigated PFGE and cgMLST clusters containing human isolates. Discrimination of isolates was significantly higher by cgMLST than by PFGE (p<0.001). cgMLST discriminated unrelated isolates that shared identical PFGE profiles and phylogenetically closely related isolates with distinct PFGE profiles. This procedure also refined epidemiologic investigations to include only phylogenetically closely related isolates, improved source identification, and facilitated epidemiologic investigations, enabling identification of more outbreaks at earlier stages. WGS-based typing should replace PFGE as the primary typing method for L. monocytogenes.
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影响因子：
11.8
作者：
Goulet V;Hedberg C;Le Monnier A;de Valk H
通讯作者：
de Valk H
影响因子：
56.3
作者：
Charlier, Caroline;Perrodeau, Elodie;Lecuit, Marc
通讯作者：
Lecuit, Marc
影响因子：
9.4
作者：
Cody, Alison J.;McCarthy, Noel D.;Maiden, Martin C. J.
通讯作者：
Maiden, Martin C. J.
影响因子：
4.4
作者：
Bratcher HB;Corton C;Jolley KA;Parkhill J;Maiden MC
通讯作者：
Maiden MC
影响因子：
11.8
作者：
Jensen, Anne Kvistholm;Nielsen, Eva Moller;Ethelberg, Steen
通讯作者：
Ethelberg, Steen