Tetraspanin Cd9b and Cxcl12a/Cxcr4b have a synergistic effect on the control of collective cell migration.
Tetraspanin Cd9b and Cxcl12a/Cxcr4b have a synergistic effect on the control of collective cell migration.
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DOI：
10.1371/journal.pone.0260372
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发表时间：
2021
期刊：
影响因子：
3.7
通讯作者：
Partridge LJ
中科院分区：
文献类型：
--
作者：
Marsay KS;Greaves S;Mahabaleshwar H;Ho CM;Roehl H;Monk PN;Carney TJ;Partridge LJ
集体细胞迁移对于胚胎发育和稳态过程是必不可少的。在斑马鱼发育过程中，后侧线原基（posterior lateral line primordium，pLLP）通过集体细胞迁移沿胚胎侧腹沿着移动。趋化因子受体Cxcr 4 b和Cxcr 7 b以及它们的同源配体Cxcl 12 a对于该过程是必需的。我们证实，敲低斑马鱼cd 9四跨膜蛋白同源物，cd 9 b，导致轻度pLL异常。通过CRISPR和TALEN突变体的产生，我们表明cd 9a和cd 9 b在pLLP迁移中部分冗余地起作用，这在cd 9 b单突变体和cd 9a; cd 9 b双突变体中被延迟。这种延迟导致神经肥大数量的短暂减少。Cd 9a和Cd 9 b两者的损失使致敏胚胎降低Cxcr 4 b和Cxcl 12 a水平。总之，这些结果提供的证据表明，镉9调制集体细胞迁移的pLLP在斑马鱼发育过程中。对这些观察结果的一种解释是Cd 9有助于更有效的趋化因子信号传导。
Collective cell migration is essential for embryonic development and homeostatic processes. During zebrafish development, the posterior lateral line primordium (pLLP) navigates along the embryo flank by collective cell migration. The chemokine receptors, Cxcr4b and Cxcr7b, as well as their cognate ligand, Cxcl12a, are essential for this process. We corroborate that knockdown of the zebrafish cd9 tetraspanin orthologue, cd9b, results in mild pLL abnormalities. Through generation of CRISPR and TALEN mutants, we show that cd9a and cd9b function partially redundantly in pLLP migration, which is delayed in the cd9b single and cd9a; cd9b double mutants. This delay led to a transient reduction in neuromast numbers. Loss of both Cd9a and Cd9b sensitized embryos to reduced Cxcr4b and Cxcl12a levels. Together these results provide evidence that Cd9 modulates collective cell migration of the pLLP during zebrafish development. One interpretation of these observations is that Cd9 contributes to more effective chemokine signalling.
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影响因子：
10.7
作者：
Brunet, Frederic G.;Roest Crollius, Hugues;Robinson-Rechavi, Marc
通讯作者：
Robinson-Rechavi, Marc
DOI：
10.1073/pnas.252339399
发表时间：
2002-12-10
影响因子：
11.1
作者：
David, NB;Sapède, D;Ghysen, A
通讯作者：
Ghysen, A
影响因子：
--
作者：
Dambly-Chaudière C;Cubedo N;Ghysen A
通讯作者：
Ghysen A
影响因子：
14.9
作者：
Howe DG;Bradford YM;Conlin T;Eagle AE;Fashena D;Frazer K;Knight J;Mani P;Martin R;Moxon SA;Paddock H;Pich C;Ramachandran S;Ruef BJ;Ruzicka L;Schaper K;Shao X;Singer A;Sprunger B;Van Slyke CE;Westerfield M
通讯作者：
Westerfield M
影响因子：
9.2
作者：
Colak-Champollion, Tugba;Lan, Ling;Knaut, Holger
通讯作者：
Knaut, Holger