Estimation of fish biomass using environmental DNA.
Estimation of fish biomass using environmental DNA.
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DOI：
10.1371/journal.pone.0035868
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Kawabata Z
中科院分区：
文献类型：
--
作者：
Takahara T;Minamoto T;Yamanaka H;Doi H;Kawabata Z
来自水生脊椎动物的环境DNA （eDNA）最近被用来估计一个物种的存在。我们假设鱼类向水中释放DNA的速度与它们的生物量相称。因此，目标物种的eDNA浓度可以用来估计物种的生物量。通过室内和野外试验，建立了一种估算鲤鱼生物量的eDNA方法。在水族箱中，eDNA浓度最初发生变化，但在6天后达到平衡。温度对水族箱内eDNA浓度没有影响。在水族馆和实验池塘中，eDNA浓度与鲤鱼生物量呈显著正相关。利用该方法对天然淡水泻湖中鲤鱼的生物量和分布进行了估算。结果表明，水温可以解释鲤鱼eDNA浓度的分布。我们的研究结果表明，从eDNA浓度估计的生物量数据反映了鲤鱼在自然环境中的潜在分布。测量eDNA浓度为估算生物量提供了一种无创、简单、快速的方法。这种方法可以为保护生态系统的管理计划提供信息。
Environmental DNA (eDNA) from aquatic vertebrates has recently been used to estimate the presence of a species. We hypothesized that fish release DNA into the water at a rate commensurate with their biomass. Thus, the concentration of eDNA of a target species may be used to estimate the species biomass. We developed an eDNA method to estimate the biomass of common carp (Cyprinus carpio L.) using laboratory and field experiments. In the aquarium, the concentration of eDNA changed initially, but reached an equilibrium after 6 days. Temperature had no effect on eDNA concentrations in aquaria. The concentration of eDNA was positively correlated with carp biomass in both aquaria and experimental ponds. We used this method to estimate the biomass and distribution of carp in a natural freshwater lagoon. We demonstrated that the distribution of carp eDNA concentration was explained by water temperature. Our results suggest that biomass data estimated from eDNA concentration reflects the potential distribution of common carp in the natural environment. Measuring eDNA concentration offers a non-invasive, simple, and rapid method for estimating biomass. This method could inform management plans for the conservation of ecosystems.
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影响因子：
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