Effect of catechins on the growth of oxygen-sensitive probiotic bacteria
Effect of catechins on the growth of oxygen-sensitive probiotic bacteria
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儿茶素对氧敏感益生菌生长的影响
DOI：
10.1016/j.foodres.2012.10.014
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发表时间：
2013
影响因子：
8.1
通讯作者：
M. Subirade
中科院分区：
文献类型：
--
作者：
H. Gaudreau;C. Champagne;G. Remondetto;L. Bazinet;M. Subirade
氧在氧敏感性细菌的生存能力丧失中起主要作用。抗氧化剂化合物，如儿茶素，可用于限制氧气暴露对细菌在食品中生长和储存期间的负面影响。本研究的目的是测量不同浓度的（+）-儿茶素水合物、绿色茶表没食子儿茶素没食子酸酯和绿色茶提取物（GTE）对具有不同氧敏感性的益生菌菌株长双歧杆菌ATCC 15708、B. longumsubspinfantisATCC 15697和瑞士乳杆菌R 0052。进行培养基的氧化还原电位（ORP）测量以及细菌细胞的傅立叶变换红外（FTIR）光谱分析以评估GTE补充的效果。所得结果表明，培养基中富含儿茶素并不刺激两种双歧杆菌的生长。但L.在有氧条件下，通过在培养基中补充GTE，helveticus的生长得到了极大的提高。这种生长促进作用可能部分是由于添加GTE后培养基的初始ORP值降低。此外，观察到的脂肪酸对应的FTIR光谱区域的差异表明GTE也对细菌细胞膜的脂质产生影响。因此，GTE补充可用于限制特定益生菌菌株的氧毒性，但生长促进作用似乎是菌株依赖性的。
Oxygen plays a major role in the loss of viability of oxygen-sensitive bacteria. Antioxidant compounds, such as catechins, could be used to limit negative effects of oxygen exposure on bacteria during their growth and storage in food products. The objective of the present study was to measure the effects of different concentrations of (+)-catechin hydrate, green tea epigallocatechin gallate and green tea extracts (GTE) on the growth of probiotic strains with different oxygen sensitivities: Bifidobacterium longum ATCC 15708,B. longumsubspinfantisATCC 15697 and Lactobacillus helveticus R0052. Redox potential (ORP) measurements of the culture medium as well as Fourier-transform infrared (FTIR) spectroscopy analyses on bacterial cells were carried out to evaluate the effects of GTE supplementation. Results obtained showed that medium enrichment with catechins did not stimulate the growth of the two bifidobacteria. However, the growth ofL. helveticuswas greatly enhanced, under aerobic conditions, by supplementation of the medium with GTE. This growth-promoting effect could be due, in part, by the reduction of the initial ORP value of the medium following addition of GTE. Moreover, the differences in the FTIR spectral region corresponding to fatty acids observed suggest an effect of GTE also on the lipids of the bacterial cell membrane. Thus, GTE supplementation could be used to limit oxygen toxicity on specific probiotic strains but the growth-promoting effect seems to be strain-dependent.
影响因子：
6.1
作者：
Uekusa, Yoshinori;Kamihira, Miya;Nakayama, Tsutomu
通讯作者：
Nakayama, Tsutomu