Protection of photosystem I during sudden light stress depends on ferredoxin:NADP(H) reductase abundance and interactions.
Protection of photosystem I during sudden light stress depends on ferredoxin:NADP(H) reductase abundance and interactions.
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DOI：
10.1093/plphys/kiab550
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发表时间：
2022-02-04
期刊：
影响因子：
7.4
通讯作者：
Hanke GT
中科院分区：
文献类型：
--
作者：
Rodriguez-Heredia M;Saccon F;Wilson S;Finazzi G;Ruban AV;Hanke GT
植物对强光和氧化胁迫的耐受性通过光合酶铁氧还蛋白：NADP（H）还原酶（FNR）的过表达而增加，但FNR介导的保护的具体机制仍然是谜。据报道，这种酶在叶绿体内的定位与其在胁迫耐受性中的作用有关。本文以拟南芥（Arabidopsis thaliana）为材料，研究了FNR含量和位置对光系统I（PSI）和光系统II（PSII）在强光胁迫下的光失活的影响。PSII的反应中心在光胁迫期间有效地翻转，而PSI的损伤需要更长的时间来修复。我们的研究结果表明PSI特异性效应，其中PSI主要供体（P700）在从黑暗过渡到光照时的有效氧化依赖于FNR向类囊体膜系链蛋白的募集：类囊体硫氰酸酶样蛋白（TROL）和叶绿体62（Tic62）的内包膜处的转位子。当这些相互作用被破坏时，PSI光失活发生。与此相反，有一个温和的延迟PSII损伤的发病。基于ΔpH形成和循环电子流的测量，我们提出FNR位置影响诱导光合控制的速度，从而对PSI损伤产生特定影响。因此，FNR的膜束缚通过调节对光的短期响应期间的电子分布而在缓解强光胁迫中起作用。改变位置的一个关键酶参与后光系统I电子传递链改善损害光系统I在增加光强度。
Plant tolerance to high light and oxidative stress is increased by overexpression of the photosynthetic enzyme Ferredoxin:NADP(H) reductase (FNR), but the specific mechanism of FNR-mediated protection remains enigmatic. It has also been reported that the localization of this enzyme within the chloroplast is related to its role in stress tolerance. Here, we dissected the impact of FNR content and location on photoinactivation of photosystem I (PSI) and photosystem II (PSII) during high light stress of Arabidopsis (Arabidopsis thaliana). The reaction center of PSII is efficiently turned over during light stress, while damage to PSI takes much longer to repair. Our results indicate a PSI sepcific effect, where efficient oxidation of the PSI primary donor (P700) upon transition from darkness to light, depends on FNR recruitment to the thylakoid membrane tether proteins: thylakoid rhodanase-like protein (TROL) and translocon at the inner envelope of chloroplasts 62 (Tic62). When these interactions were disrupted, PSI photoinactivation occurred. In contrast, there was a moderate delay in the onset of PSII damage. Based on measurements of ΔpH formation and cyclic electron flow, we propose that FNR location influences the speed at which photosynthetic control is induced, resulting in specific impact on PSI damage. Membrane tethering of FNR therefore plays a role in alleviating high light stress, by regulating electron distribution during short-term responses to light. Altered location of a key enzyme involved in the post-photosystem I electron transport chain ameliorates damage to photosystem I during increasing light intensity.
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影响因子：
7.2
作者：
Hashimoto, M;Endo, T;Shikanai, T
通讯作者：
Shikanai, T
影响因子：
7.2
作者：
Juric, Snjezana;Hazler-Pilepic, Kroata;Fulgosi, Hrvoje
通讯作者：
Fulgosi, Hrvoje
影响因子：
3.5
作者：
CLARK, RD;HAWKESFORD, MJ;HIND, G
通讯作者：
HIND, G
影响因子：
3.5
作者：
Fufezan, C;Rutherford, AW;Krieger-Liszkay, A
通讯作者：
Krieger-Liszkay, A
影响因子：
4.8
作者：
Johnson, Matthew P.;Ruban, Alexander V.
通讯作者：
Ruban, Alexander V.