Assessing the Covalent Attachment and Energy Transfer Capabilities of Upconverting Phosphors With Cofactor Containing Bioactive Enzymes.
Assessing the Covalent Attachment and Energy Transfer Capabilities of Upconverting Phosphors With Cofactor Containing Bioactive Enzymes.
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DOI：
10.3389/fchem.2020.613334
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发表时间：
2020
影响因子：
5.5
通讯作者：
Natrajan LS
中科院分区：
文献类型：
--
作者：
Burgess L;Wilson H;Jones AR;Hay S;Natrajan LS
上转换荧光粉(UCP)通过光子上转换过程将多个低能光子转化为较高的能量发射，为有机荧光团作为发光探针提供了一种有吸引力的替代方案。利用UCP和纳米磷光体(UCNPs)与生物分子的表观能量转移的生物传感器的例子已经开始出现在文献中，但很少有人利用生物分子的共价锚定到UCP表面来提高系统的灵敏度。在这里，我们展示了一种稳健和通用的方法，将生物分子共价连接到各种UCP和UCNP的表面，其中UCPs被功能化的二氧化硅覆盖，以便提供一个表面，使生物分子与表面可达的半胱氨酸共价连接。然后，通过马来酰亚胺-硫醇偶联将BM3heme、细胞色素C、葡萄糖氧化酶和谷胱甘肽还原酶的变体连接起来。BM3heme、葡萄糖氧化酶和谷胱甘肽还原酶在与UCPs结合时保持了它们的活性，可能为生物传感应用开辟了机会。
Upconverting phosphors (UCPs) convert multiple low energy photons into higher energy emission via the process of photon upconversion and offer an attractive alternative to organic fluorophores for use as luminescent probes. Examples of biosensors utilizing the apparent energy transfer of UCPs and nanophosphors (UCNPs) with biomolecules have started to appear in the literature but very few exploit the covalent anchoring of the biomolecule to the surface of the UCP to improve the sensitivity of the systems. Here, we demonstrate a robust and versatile method for the covalent attachment of biomolecules to the surface of a variety of UCPs and UCNPs in which the UCPs were capped with functionalized silica in order to provide a surface to covalently conjugate biomolecules with surface-accessible cysteines. Variants of BM3Heme, cytochrome C, glucose oxidase, and glutathione reductase were then attached via maleimide-thiol coupling. BM3Heme, glucose oxidase, and glutathione reductase were shown to retain their activity when coupled to the UCPs potentially opening up opportunities for biosensing applications.
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影响因子：
14
作者：
Chatteriee, Dev K.;Rufalhah, Abdul J.;Zhang, Yong
通讯作者：
Zhang, Yong
DOI：
10.3390/s17081866
发表时间：
2017-08-12
期刊：
Sensors (Basel, Switzerland)
影响因子：
--
作者：
Bruen D;Delaney C;Florea L;Diamond D
通讯作者：
Diamond D
影响因子：
3.3
作者：
Blackburn, Octavia A.;Tropiano, Manuel;Faulkner, Stephen
通讯作者：
Faulkner, Stephen
影响因子：
3
作者：
Can, Betul;Kulaksiz Erkmen, Gulnihal;Ozer, Nazmi
通讯作者：
Ozer, Nazmi
影响因子：
4.8
作者：
Girvan, Hazel M.;Seward, Harriet E.;Munro, Andrew W.
通讯作者：
Munro, Andrew W.