Netosis and Inflammasomes in Large Vessel Occlusion Thrombi.
Netosis and Inflammasomes in Large Vessel Occlusion Thrombi.
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DOI：
10.3389/fphar.2020.607287
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发表时间：
2020
影响因子：
5.6
通讯作者：
de Rivero Vaccari JP
中科院分区：
文献类型：
--
作者：
Chen SH;Scott XO;Ferrer Marcelo Y;Almeida VW;Blackwelder PL;Yavagal DR;Peterson EC;Starke RM;Dietrich WD;Keane RW;de Rivero Vaccari JP
The inflammatory response appears to play a critical role in clotting in which neutrophil extracellular traps (NETs) are the major drivers of thrombosis in acute ischemic stroke (AIS). The inflammasome is an innate immune complex involved in the activation of interleukin (IL)-18 and IL-1β through caspase-1, but whether the inflammasome plays a role in NETosis in AIS remains poorly understood. Here we assessed the levels of inflammasome signaling proteins in NETs and their association with clinical and procedural outcomes of mechanical thrombectomy for AIS. Electron microscopy and immunofluorescence indicate the presence of NETs in thrombi of patients with AIS. Moreover, the inflammasome signaling proteins caspase-1 and apoptosis-associated speck-like protein containing a caspase recruitment domain (ASC) were also present in clots associated with the marker of NETosis citrullinated histone 3H (CitH3). Analysis of protein levels by a simple plex assay show that caspase-1, ASC and interleukin (IL)-1β were significantly elevated in clots when compared to plasma of AIS patients and healthy controls, while IL-18 levels were lower. Moreover, multivariate analyses show that IL-1β levels in clots contribute to the number of passes to achieve complete recanalization, and that ASC, caspase-1 and IL-18 are significant contributors to time to recanalization. Thus, inflammasome proteins are elevated in NETs present in thrombi of patients with AIS that contribute to poor outcomes following stroke.
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影响因子：
2.5
作者：
Fransen PS;Beumer D;Berkhemer OA;van den Berg LA;Lingsma H;van der Lugt A;van Zwam WH;van Oostenbrugge RJ;Roos YB;Majoie CB;Dippel DW;MR CLEAN Investigators
通讯作者：
MR CLEAN Investigators
影响因子：
4.6
作者：
Ismael S;Zhao L;Nasoohi S;Ishrat T
通讯作者：
Ishrat T
影响因子：
3.7
作者：
Hirose T;Hamaguchi S;Matsumoto N;Irisawa T;Seki M;Tasaki O;Hosotsubo H;Yamamoto N;Yamamoto K;Akeda Y;Oishi K;Tomono K;Shimazu T
通讯作者：
Shimazu T
影响因子：
4.6
作者：
Bester J;Pretorius E
通讯作者：
Pretorius E
DOI：
10.1186/s12950-016-0137-0
发表时间：
2016
期刊：
Journal of inflammation (London, England)
影响因子：
--
作者：
Brand FJ 3rd;Forouzandeh M;Kaur H;Travascio F;de Rivero Vaccari JP
通讯作者：
de Rivero Vaccari JP