Isolation and characterization of extracellular vesicles produced by cell lines.
Isolation and characterization of extracellular vesicles produced by cell lines.
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DOI：
10.1016/j.xpro.2021.100295
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发表时间：
2021-03-19
期刊：
影响因子：
--
通讯作者：
Antonyak MA
中科院分区：
文献类型：
--
作者：
Wang F;Cerione RA;Antonyak MA
细胞产生两大类细胞外囊泡（EV），外泌体和微囊泡（MV）。外来体是源自多泡体的30-150 nm囊泡，而MV是从质膜夹断的200- 1，000 nm囊泡。EV的可靠分离对于了解其生化和功能特性至关重要。在这里，我们描述了一个协议，从哺乳动物细胞系的条件培养基中分离和表征EV。该方案已针对贴壁细胞进行了优化，但也可适用于悬浮细胞。有关本方案使用和执行的完整详细信息，请参阅。细胞产生两大类细胞外囊泡，外泌体和微囊泡细胞外囊泡作为细胞间通讯的介体。EV的内容物促进表型变化，并可用作诊断标志物。在这里，我们提供了一种用于分离外泌体和微囊泡的优化方案。细胞产生两大类细胞外囊泡（EV），外泌体和微囊泡（MV）。外来体是源自多泡体的30-150 nm囊泡，而MV是从质膜夹断的200- 1，000 nm囊泡。EV的可靠分离对于了解其生化和功能特性至关重要。在这里，我们描述了一个协议，从哺乳动物细胞系的条件培养基中分离和表征EV。该方案已针对贴壁细胞进行了优化，但也可适用于悬浮细胞。
Cells produce two broad classes of extracellular vesicles (EVs), exosomes and microvesicles (MVs). Exosomes are 30–150 nm vesicles derived from multivesicular bodies, while MVs are 200–1,000 nm vesicles that pinch off from plasma membranes. Reliable isolation of EVs is crucial to understand their biochemical and functional properties. Here, we describe a protocol to isolate and characterize EVs from conditioned medium from mammalian cell lines. This protocol has been optimized for adherent cells but can also be adapted for suspension cells. For complete details on the use and execution of this protocol, please refer to. Cells produce two major classes of extracellular vesicles, exosomes and microvesicles Extracellular vesicles function as mediators of intercellular communication The contents of EVs promote phenotypic changes and can be used as diagnostic markers Here, we provide an optimized protocol for isolating exosomes and microvesicles Cells produce two broad classes of extracellular vesicles (EVs), exosomes and microvesicles (MVs). Exosomes are 30–150 nm vesicles derived from multivesicular bodies, while MVs are 200–1,000 nm vesicles that pinch off from plasma membranes. Reliable isolation of EVs is crucial to understand their biochemical and functional properties. Here, we describe a protocol to isolate and characterize EVs from conditioned medium from mammalian cell lines. This protocol has been optimized for adherent cells but can also be adapted for suspension cells.
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影响因子：
4.6
作者：
Willms E;Johansson HJ;Mäger I;Lee Y;Blomberg KE;Sadik M;Alaarg A;Smith CI;Lehtiö J;El Andaloussi S;Wood MJ;Vader P
通讯作者：
Vader P
DOI：
10.1073/pnas.1017667108
发表时间：
2011-03-22
影响因子：
11.1
作者：
Antonyak, Marc A.;Li, Bo;Cerione, Richard A.
通讯作者：
Cerione, Richard A.
影响因子：
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作者：
Latifkar, Arash;Ling, Lu;Antonyak, Marc A.
通讯作者：
Antonyak, Marc A.
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影响因子：
32.4
作者：
Zhang, Fanghui;Li, Rongrong;Cai, Zhijian
通讯作者：
Cai, Zhijian
影响因子：
64.5
作者：
Jeppesen, Dennis K.;Fenix, Aidan M.;Coffey, Robert J.
通讯作者：
Coffey, Robert J.