The Influence of B Cell Depletion Therapy on Naturally Acquired Immunity to Streptococcus pneumoniae.
The Influence of B Cell Depletion Therapy on Naturally Acquired Immunity to Streptococcus pneumoniae.
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DOI：
10.3389/fimmu.2020.611661
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发表时间：
2020
影响因子：
7.3
通讯作者：
Brown J
中科院分区：
文献类型：
--
作者：
Ercoli G;Ramos-Sevillano E;Nakajima R;de Assis RR;Jasinskas A;Goldblatt D;Felgner P;Weckbecker G;Brown J
抗CD20抗体利妥昔单抗消耗CD20 + B细胞是类风湿性关节炎和B细胞恶性肿瘤的有效治疗方法，但与呼吸道感染的发病率增加相关。使用小鼠模型，我们研究了B细胞耗竭对肺炎链球菌的天然和获得性体液免疫的影响。幼稚C57 B1/6小鼠的B细胞耗竭降低了S. pneumoniae，但不增加对S.肺炎ELISA和流式细胞术检测表明，S.在S. pneumoniae感染前用B细胞去除处理的小鼠血清中，与未处理的小鼠相比，肺炎链球菌鼻咽定殖。定殖诱导对蛋白质而不是荚膜抗原的抗体应答，并且当在定殖之前使用蛋白质阵列B细胞耗竭进行测量时，降低了针对几种蛋白质抗原的IgG的血清水平。而B细胞对S. pneumoniae定殖的小鼠在用S.肺炎后重建其B细胞。这些数据表明，尽管B细胞耗竭显著损害了S.在定殖小鼠中，维持了一些保护性免疫，可能是由细胞免疫介导的。
The anti-CD20 antibody Rituximab to deplete CD20+ B cells is an effective treatment for rheumatoid arthritis and B cell malignancies, but is associated with an increased incidence of respiratory infections. Using mouse models we have investigated the consequences of B cell depletion on natural and acquired humoral immunity to Streptococcus pneumoniae. B cell depletion of naïve C57Bl/6 mice reduced natural IgM recognition of S. pneumoniae, but did not increase susceptibility to S. pneumoniae pneumonia. ELISA and flow cytometry assays demonstrated significantly reduced IgG and IgM recognition of S. pneumoniae in sera from mice treated with B cell depletion prior to S. pneumoniae nasopharyngeal colonization compared to untreated mice. Colonization induced antibody responses to protein rather than capsular antigen, and when measured using a protein array B cell depletion prior to colonization reduced serum levels of IgG to several protein antigens. However, B cell depleted S. pneumoniae colonized mice were still partially protected against both lung infection and septicemia when challenged with S. pneumoniae after reconstitution of their B cells. These data indicate that although B cell depletion markedly impairs antibody recognition of S. pneumoniae in colonized mice, some protective immunity is maintained, perhaps mediated by cellular immunity.
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DOI：
10.1084/jem.20161621
发表时间：
2017-05-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Deniset JF;Surewaard BG;Lee WY;Kubes P
通讯作者：
Kubes P
DOI：
10.4049/jimmunol.1201427
发表时间：
2013-02-01
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Horikawa M;Weimer ET;DiLillo DJ;Venturi GM;Spolski R;Leonard WJ;Heise MT;Tedder TF
通讯作者：
Tedder TF
影响因子：
56.9
作者：
Garside, P;Ingulli, E;Jenkins, MK
通讯作者：
Jenkins, MK
影响因子：
9.1
作者：
Eisenberg RA;Jawad AF;Boyer J;Maurer K;McDonald K;Prak ET;Sullivan KE
通讯作者：
Sullivan KE
DOI：
10.4049/jimmunol.1101844
发表时间：
2011-11-01
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Hamel KM;Cao Y;Ashaye S;Wang Y;Dunn R;Kehry MR;Glant TT;Finnegan A
通讯作者：
Finnegan A