Structure of nucleosome-bound human PBAF complex.
Structure of nucleosome-bound human PBAF complex.
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DOI：
10.1038/s41467-022-34859-5
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发表时间：
2022-12-10
影响因子：
16.6
通讯作者：
Xu, Yanhui
中科院分区：
文献类型：
--
作者：
Wang, Li;Yu, Jiali;Yu, Zishuo;Wang, Qianmin;Li, Wanjun;Ren, Yulei;Chen, Zhenguo;He, Shuang;Xu, Yanhui
BAF和PBAF是哺乳动物SWI/SNF家族染色质重塑复合物，具有多个组蛋白/DNA结合亚基，并产生用于转录激活的核小体耗尽/游离区域。尽管对SWI/SNF家族复合物的结构研究和最新进展，但PBAF-核小体复合物是如何组织的仍不完全清楚。在这里，我们确定了ADP-BeF 3结合状态下与乙酰化核小体复合的13亚基人PBAF的结构。四个PBAF特异性亚基与九个BAF/PBAF共享亚基一起工作，产生PBAF特异性模块化组织，与不同区域的BAF不同。PBAF-核小体结构揭示了6个组蛋白结合结构域和4个DNA结合结构域/模块，其中大部分直接结合组蛋白/DNA。这种多价核小体结合模式，在以前的研究中没有观察到，这表明PBAF可以整合全面的染色质信息，以靶向基因组位点的功能。我们的研究揭示了PBAF-核小体复合物中亚基和组蛋白/DNA结合结构域/模块的分子组织，并为PBAF介导的核小体缔合提供了与最近报道的PBAF-核小体结构互补的结构见解。BAF和PBAF是SWI/SNF家族染色质重塑复合物，其产生用于转录激活的核小体耗尽区域。在这里，作者报告了13亚基人PBAF与乙酰化核小体在ADP-BeF 3结合状态下的复合物的结构，这为PBAF介导的核小体缔合提供了结构上的见解。
BAF and PBAF are mammalian SWI/SNF family chromatin remodeling complexes that possess multiple histone/DNA-binding subunits and create nucleosome-depleted/free regions for transcription activation. Despite previous structural studies and recent advance of SWI/SNF family complexes, it remains incompletely understood how PBAF-nucleosome complex is organized. Here we determined structure of 13-subunit human PBAF in complex with acetylated nucleosome in ADP-BeF3-bound state. Four PBAF-specific subunits work together with nine BAF/PBAF-shared subunits to generate PBAF-specific modular organization, distinct from that of BAF at various regions. PBAF-nucleosome structure reveals six histone-binding domains and four DNA-binding domains/modules, the majority of which directly bind histone/DNA. This multivalent nucleosome-binding pattern, not observed in previous studies, suggests that PBAF may integrate comprehensive chromatin information to target genomic loci for function. Our study reveals molecular organization of subunits and histone/DNA-binding domains/modules in PBAF-nucleosome complex and provides structural insights into PBAF-mediated nucleosome association complimentary to the recently reported PBAF-nucleosome structure. BAF and PBAF are SWI/SNF family chromatin remodeling complexes that create nucleosome-depleted regions for transcription activation. Here the authors report the structure of a 13-subunit human PBAF in complex with acetylated nucleosome in ADP-BeF3-bound state that provides structural insights into PBAF-mediated nucleosome association.
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影响因子：
48
作者：
Kastner, Berthold;Fischer, Niels;Stark, Holger
通讯作者：
Stark, Holger
哺乳动物SWI/SNF复合物的蛋白质组学和生物信息学分析确定了在人类恶性肿瘤中的广泛作用。
影响因子：
30.8
作者：
Kadoch, Cigall;Hargreaves, Diana C.;Hodges, Courtney;Elias, Laura;Ho, Lena;Ranish, Jeff;Crabtree, Gerald R.
通讯作者：
Crabtree, Gerald R.
影响因子：
16
作者：
Badis, Gwenael;Chan, Esther T.;van Bakel, Harm;Pena-Castillo, Lourdes;Tillo, Desiree;Tsui, Kyle;Carlson, Clayton D.;Gossett, Andrea J.;Hasinoff, Michael J.;Warren, Christopher L.;Gebbia, Marinella;Talukder, Shaheynoor;Yang, Ally;Mnaimneh, Sanie;Terterov, Dimitri;Coburn, David;Yeo, Ai Li;Yeo, Zhen Xuan;Clarke, Neil D.;Lieb, Jason D.;Ansari, Aseem Z.;Nislow, Corey;Hughes, Timothy R.
通讯作者：
Hughes, Timothy R.
DOI：
10.1107/s0907444904019158
发表时间：
2004-12-01
影响因子：
2.2
作者：
Emsley, P;Cowtan, K
通讯作者：
Cowtan, K
影响因子：
56.9
作者：
Chen, Xizi;Qi, Yilun;Xu, Yanhui
通讯作者：
Xu, Yanhui