A library of yeast transcription factor motifs reveals a widespread function for Rsc3 in targeting nucleosome exclusion at promoters.
A library of yeast transcription factor motifs reveals a widespread function for Rsc3 in targeting nucleosome exclusion at promoters.
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DOI：
10.1016/j.molcel.2008.11.020
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发表时间：
2008-12-26
期刊：
影响因子：
16
通讯作者：
Hughes, Timothy R.
中科院分区：
文献类型：
--
作者：
Badis, Gwenael;Chan, Esther T.;van Bakel, Harm;Pena-Castillo, Lourdes;Tillo, Desiree;Tsui, Kyle;Carlson, Clayton D.;Gossett, Andrea J.;Hasinoff, Michael J.;Warren, Christopher L.;Gebbia, Marinella;Talukder, Shaheynoor;Yang, Ally;Mnaimneh, Sanie;Terterov, Dimitri;Coburn, David;Yeo, Ai Li;Yeo, Zhen Xuan;Clarke, Neil D.;Lieb, Jason D.;Ansari, Aseem Z.;Nislow, Corey;Hughes, Timothy R.
DNA结合蛋白的序列特异性是细胞识别基因组特征的主要机制。在这里，我们描述了酵母转录因子DNA结合特异性的系统测定。我们获得了112个DNA结合蛋白的结合特异性，代表19个不同的结构类别，其中三分之一以前没有报道过。几个新发现的结合序列有显着的基因组分布相对于转录起始位点，支持其生物相关性，并建议在启动子结构中的作用。其中包括Rsc 3结合序列，其含有核心CGCG，其优选在转录起始位点上游约100 bp处发现。RSC 3的突变导致在数百个含有Rsc 3结合元件的近端启动子中核小体占据率的急剧增加，但对缺乏Rsc 3结合序列的启动子几乎没有影响，表明Rsc 3在酵母启动子处靶向核小体排斥中起着广泛的作用。
The sequence specificity of DNA-binding proteins is the primary mechanism by which the cell recognizes genomic features. Here, we describe systematic determination of yeast transcription factor DNA-binding specificities. We obtained binding specificities for 112 DNA-binding proteins representing 19 distinct structural classes, one-third of which have not been previously reported. Several newly discovered binding sequences have striking genomic distributions relative to transcription start sites, supporting their biological relevance and suggesting a role in promoter architecture. Among these are Rsc3 binding sequences, containing the core CGCG, which are found preferentially ~100 bp upstream of transcription start sites. Mutation of RSC3 results in a dramatic increase in nucleosome occupancy in hundreds of proximal promoters containing a Rsc3 binding element, but has little impact on promoters lacking Rsc3 binding sequences, indicating that Rsc3 plays a broad role in targeting nucleosome exclusion at yeast promoters.
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