The mitochondrial phosphate transporters modulate plant responses to salt stress via affecting ATP and gibberellin metabolism in Arabidopsis thaliana.
The mitochondrial phosphate transporters modulate plant responses to salt stress via affecting ATP and gibberellin metabolism in Arabidopsis thaliana.
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DOI：
10.1371/journal.pone.0043530
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Zheng C
中科院分区：
文献类型：
--
作者：
Zhu W;Miao Q;Sun D;Yang G;Wu C;Huang J;Zheng C
线粒体磷酸盐转运蛋白（MPT）在植物细胞ATP合成中起着重要作用。在拟南芥中已经鉴定出三个MPT基因。本文报道了高盐胁迫下拟南芥AtMPTs的mRNA表达量上调。拟南芥幼苗在种子萌发和成苗阶段，过表达AtMPTs的转基因植株对盐胁迫的敏感性均高于野生型植株。过量表达植株的ATP含量和能荷均高于野生型A。盐胁迫下的拟南芥因此，过量表达的植株在外源施用甘草苷后，由于ATP含量的变化而恢复了盐敏感表型。Genevestigator的调查和qRT-PCR分析表明，包括赤霉素合成相关基因在内的大量基因在逆境条件下受到能量可利用性变化的调控。thaliana.此外，外源烯效唑的过度表达线表明，赤霉素的稳态被打乱的过度表达。我们的研究揭示了AtMPTs介导的ATP含量与赤霉素代谢在高盐胁迫下的反应之间可能存在联系。thaliana.
The mitochondrial phosphate transporter (MPT) plays crucial roles in ATP production in plant cells. Three MPT genes have been identified in Arabidopsis thaliana. Here we report that the mRNA accumulations of AtMPTs were up-regulated by high salinity stress in A. thaliana seedlings. And the transgenic lines overexpressing AtMPTs displayed increased sensitivity to salt stress compared with the wild-type plants during seed germination and seedling establishment stages. ATP content and energy charge was higher in overexpressing plants than those in wild-type A. thaliana under salt stress. Accordingly, the salt-sensitive phenotype of overexpressing plants was recovered after the exogenous application of atractyloside due to the change of ATP content. Interestingly, Genevestigator survey and qRT-PCR analysis indicated a large number of genes, including those related to gibberellin synthesis could be regulated by the energy availability change under stress conditions in A. thaliana. Moreover, the exogenous application of uniconazole to overexpressing lines showed that gibberellin homeostasis was disturbed in the overexpressors. Our studies reveal a possible link between the ATP content mediated by AtMPTs and gibberellin metabolism in responses to high salinity stress in A. thaliana.
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