Comparative analysis of naive, primed and ground state pluripotency in mouse embryonic stem cells originating from the same genetic background.
Comparative analysis of naive, primed and ground state pluripotency in mouse embryonic stem cells originating from the same genetic background.
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DOI：
10.1038/s41598-018-24051-5
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发表时间：
2018-04-12
影响因子：
4.6
通讯作者：
Heindryckx B
中科院分区：
文献类型：
--
作者：
Ghimire S;Van der Jeught M;Neupane J;Roost MS;Anckaert J;Popovic M;Van Nieuwerburgh F;Mestdagh P;Vandesompele J;Deforce D;Menten B;Chuva de Sousa Lopes S;De Sutter P;Heindryckx B
小鼠胚胎干细胞（mESC）以多能性的幼稚、致敏和基态存在。虽然已经报道了这些多能性状态的比较分析，但是所使用的mESC源自不同的遗传背景并且在不同的实验室中衍生。在常规LIF +血清培养条件下的mESC衍生是菌株依赖性的，不同的遗传背景可能影响随后的干细胞特征。在本研究中，我们进行了全面的表征幼稚，引发和基态的胚胎干细胞起源于我们的实验室内相同的遗传背景，通过比较他们的转录谱。我们显示了幼稚、引发和基态mESC的独特转录谱。虽然幼稚和基态mESC具有更相似但不相同的特征，但引发状态mESC显示出非常不同的特征。我们进一步证明了mESC向特定胚层的分化倾向高度依赖于它们各自的多能性状态。
Mouse embryonic stem cells (mESCs) exist in a naive, primed and ground state of pluripotency. While comparative analyses of these pluripotency states have been reported, the mESCs utilized originated from various genetic backgrounds and were derived in different laboratories. mESC derivation in conventional LIF + serum culture conditions is strain dependent, with different genetic backgrounds potentially affecting subsequent stem cell characteristics. In the present study, we performed a comprehensive characterization of naive, primed and ground state mESCs originating from the same genetic background within our laboratory, by comparing their transcriptional profiles. We showed unique transcriptional profiles for naive, primed and ground state mESCs. While naive and ground state mESCs have more similar but not identical profiles, primed state mESCs show a very distinct profile. We further demonstrate that the differentiation propensity of mESCs to specific germ layers is highly dependent on their respective state of pluripotency.
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影响因子：
64.8
作者：
Brons, I. Gabrielle M.;Smithers, Lucy E.;Vallier, Ludovic
通讯作者：
Vallier, Ludovic
影响因子：
64.8
作者：
Ying, Qi-Long;Wray, Jason;Smith, Austin
通讯作者：
Smith, Austin
影响因子：
64.8
作者：
Tesar, Paul J.;Chenoweth, Josh G.;McKay, Ronald D. G.
通讯作者：
McKay, Ronald D. G.
影响因子：
14.8
作者：
Huang, Da Wei;Sherman, Brad T.;Lempicki, Richard A.
通讯作者：
Lempicki, Richard A.
DOI：
10.1073/pnas.78.12.7634
发表时间：
1981-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
MARTIN, GR
通讯作者：
MARTIN, GR