Microbial Consortia and Mixed Plastic Waste: Pangenomic Analysis Reveals Potential for Degradation of Multiple Plastic Types via Previously Identified PET Degrading Bacteria.
Microbial Consortia and Mixed Plastic Waste: Pangenomic Analysis Reveals Potential for Degradation of Multiple Plastic Types via Previously Identified PET Degrading Bacteria.
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微生物联合体和混合塑料废物：泛基因组分析揭示了通过先前已确定的PET降解菌降解多种塑料类型的潜力。
DOI：
10.3390/ijms23105612
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发表时间：
2022-05-17
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
一次性不可生物降解塑料的全球使用，如由聚对苯二甲酸乙二醇酯(PET)制成的瓶子，导致了灾难性的塑料污染水平。幸运的是，微生物群落正在适应吸收塑料垃圾。此前，我们的工作显示了一个由五个细菌组成的完整联盟，能够协同降解PET。使用组学方法，我们在该联盟的pangenome和转录组中确定了与PET降解有关的关键基因。这种分析导致了一种新的PETase的发现，ESTB已被观察到能水解寡聚体BHET和聚合物PET。除了与PET降解有关的基因外，还发现了许多其他的生物降解基因。通过塑料微生物生物降解数据库(PMBD)发现了200多个与塑料和增塑剂降解有关的基因。通过基于微生物群落的分析，观察到不同的碳源利用，再加上大量的塑料和增塑剂降解酶，表明混合塑料降解的可能性很大。利用RNAseq差异分析，预测了几个与PET降解有关的基因，包括乙醛脱氢酶和几类水解酶。还观察到PET单体代谢的活跃转录，包括聚羟基烷酸(PHA)/聚羟基丁酸酯(PHB)生物聚合物的产生。这些结果为具有上行循环潜力的混合塑料废物的生物回收提供了一个令人兴奋的机会。
The global utilization of single-use, non-biodegradable plastics, such as bottles made of polyethylene terephthalate (PET), has contributed to catastrophic levels of plastic pollution. Fortunately, microbial communities are adapting to assimilate plastic waste. Previously, our work showed a full consortium of five bacteria capable of synergistically degrading PET. Using omics approaches, we identified the key genes implicated in PET degradation within the consortium’s pangenome and transcriptome. This analysis led to the discovery of a novel PETase, EstB, which has been observed to hydrolyze the oligomer BHET and the polymer PET. Besides the genes implicated in PET degradation, many other biodegradation genes were discovered. Over 200 plastic and plasticizer degradation-related genes were discovered through the Plastic Microbial Biodegradation Database (PMBD). Diverse carbon source utilization was observed by a microbial community-based assay, which, paired with an abundant number of plastic- and plasticizer-degrading enzymes, indicates a promising possibility for mixed plastic degradation. Using RNAseq differential analysis, several genes were predicted to be involved in PET degradation, including aldehyde dehydrogenases and several classes of hydrolases. Active transcription of PET monomer metabolism was also observed, including the generation of polyhydroxyalkanoate (PHA)/polyhydroxybutyrate (PHB) biopolymers. These results present an exciting opportunity for the bio-recycling of mixed plastic waste with upcycling potential.
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影响因子：
13.6
作者：
Geyer R;Jambeck JR;Law KL
通讯作者：
Law KL
影响因子：
5.2
作者：
Bonifer, Kyle S.;Wen, Xianfang;Reynolds, Todd B.
通讯作者：
Reynolds, Todd B.
AfterQC：fastq数据的自动过滤、修剪、错误消除和质量控制
影响因子：
3
作者：
Chen S;Huang T;Zhou Y;Han Y;Xu M;Gu J
通讯作者：
Gu J
影响因子：
6.4
作者：
Gambarini V;Pantos O;Kingsbury JM;Weaver L;Handley KM;Lear G
通讯作者：
Lear G
影响因子：
6.4
作者：
Blaustein, Ryan A.;McFarland, Alexander G.;Hartmann, Erica M.
通讯作者：
Hartmann, Erica M.