Genetic analysis of Bacillus subtilis spo mutations generated by Tn917-mediated insertional mutagenesis.
Genetic analysis of Bacillus subtilis spo mutations generated by Tn917-mediated insertional mutagenesis.
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Tn917 介导的插入诱变产生的枯草芽孢杆菌 spo 突变的遗传分析。
DOI：
10.1093/genetics/117.4.603
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发表时间：
1987
期刊：
影响因子：
3.3
通讯作者：
Youngman,P
中科院分区：
文献类型：
--
作者：
Sandman,K;Losick,R;Youngman,P
导致孢子形成缺陷的突变(SPO突变)通常识别发育调节的转录单位或基因，其产物是孢子形成特异性调节基因的表达所必需的。我们报道了转座子Tn917插入突变产生的突变体的分离、遗传分析和表型特征，转座子Tn917是一种形式的突变，它在许多方面促进了对突变基因的遗传和物理操作。对24个插入的$10突变进行了详细研究。根据转化和转导的连锁分析和一系列表型测试，这些突变被分配到20个不同的座位，其中至少有9个与先前描述的40个座位不同。插入突变在孢子形成的各个不同阶段造成阻塞，因此可能识别在孢子形成过程中不同时间活跃的基因。除了大幅增加已知基因的总数外，我们预计这个集合还将包括许多时间调控的基因集的代表，这些基因组成了枯草杆菌芽胞形成特异性基因激活的整体程序。考虑到Tn917插入可能对基因进行各种操作，这应该会极大地促进理解这些基因集的调控。
Mutations that cause sporulation defects (spo mutations) often identify developmentally regulated transcription units or genes whose products are required for the expression of sporulation-specific regulons. We report here the isolation, genetic analysis and phenotypic characterization ofspomutations produced by insertional mutagenesis with transposon Tn917, a form of mutagenesis that facilitates genetic and physical manipulation of mutated genes in many ways. Twenty-four insertional $10 mutations were studied in detail. On the basis of transformation-mediated and transductionmediated linkage analysis and a range of phenotypic tests, these mutations were assigned to 20 distinct loci, at least 9 of which are different from the 40 previously describedspoloci. The insertional mutations caused blocks at a variety of different stages of sporulation, and therefore probably identify genes active at different times during sporulation. In addition to increasing substantially the total of knownspoloci, we anticipate that this collection will include representatives of many of the temporally regulated sets of genes that comprise the overall program of sporulation-specific gene activation inBacillus subtilis. Given the kinds of manipulations that are possible with genes disrupted by Tn917 insertions, this should significantly facilitate efforts to understand the regulation of these gene sets.
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孢子形成和抗生素、外酶和外渗素的产生。
影响因子：
--
作者：
P. Schaeffer
通讯作者：
P. Schaeffer
DOI：
10.1099/00221287-71-1-17
发表时间：
1972
期刊：
Journal of general microbiology
影响因子：
--
作者：
J. Coote
通讯作者：
J. Coote
研究孢子形成细菌发育基因的新方法
影响因子：
56.9
作者：
P. Youngman;P. Zuber;J. Perkins;K. Sandman;M. Igo;R. Losick
通讯作者：
R. Losick
影响因子：
5.6
作者：
DONOVAN, W;ZHENG, L;LOSICK, R
通讯作者：
LOSICK, R
枯草芽孢杆菌孢子形成特异操纵子总数的统计估计
影响因子：
3.2
作者：
D. Hranueli;P. Piggot;J. Mandelstam
通讯作者：
J. Mandelstam