The influence of overexpressions of microRNA-375 on the expression of thymic stromal lymphopoietin and IL-4, IL-13 in allergic rhinitis mice
The influence of overexpressions of microRNA-375 on the expression of thymic stromal lymphopoietin and IL-4, IL-13 in allergic rhinitis mice
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microRNA-375过表达对变应性鼻炎小鼠胸腺基质淋巴细胞生成素及IL-4、IL-13表达的影响
DOI：
10.4103/1995-7645.243111
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发表时间：
2018-10
影响因子：
3.1
通讯作者：
Zhonglin Mu
中科院分区：
文献类型：
--
作者：
Yilong Wang;Zhonglin Mu
目的：通过microRNA-375过表达检测变应性鼻炎（AR）小鼠胸腺基质淋巴细胞生成素（TSLP）及白细胞介素-4、白细胞介素-13（IL-4、IL-13）的表达。研究方法：用卵清蛋白（OVA）诱导小鼠AR模型，分别设慢病毒过表达载体AR组（Overexpression vector+OVA）、慢病毒对照载体AR组（Control vector+OVA）和AR（OVA）组。对照组给予生理盐水，收集各组鼻粘膜标本备用。RT-PCR和Western blot检测TSLP和IL-4、IL-13 mRNA和蛋白的相对表达。结果如下：实时荧光定量法证实变应性鼻炎组miRNA 375过表达，过表达组IL-4、IL-13基因表达采用同样方法。microRNA-375过表达组鼻黏膜TSLP和IL-4、IL-13 mRNA及蛋白的相对表达量较AR组降低（P<0.05）。结论：结果提示，慢病毒在变应性鼻炎小鼠鼻黏膜中的表达可能影响IL-4、IL-13和TSLP的表达，且慢病毒在变应性鼻炎小鼠鼻黏膜中的表达与miR-375慢病毒组不同。慢病毒在变应性鼻炎小鼠鼻黏膜中的表达存在差异。
Objective: To detect the expression of thymic stromal lymphopoietin (TSLP) and IL-4, IL- 13 (interleukin-4, interleukin-13) through overexpression of microRNA-375 in allergic rhinitis (AR) mice. Methods: AR mice models with overexpression of miRNA375 were induced by ovalbumin (OVA) in Lentivirus overexpression vector AR group (Overexpression vector+OVA), lentivirus control vector AR group (Control vector+OVA) as well as AR (OVA) group. While saline was administered in control (Saline) group, nasal mucosa of each group were collected for further use. Relative expressions of TSLP and IL-4, IL-13 mRNA and proteins were detected by quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and western blot analysis. Results: Real-time fluorescence quantitative method verified the overexpression of miRNA375 in allergic rhinitis group, and the same method has been adopted to verify the expressions of IL-4 and IL-13 genes in the overexpression group. The relative expressions of TSLP and IL-4, IL-13 mRNA and protein decreased in overexpression of microRNA-375 nasal mucosa compared with AR group (P<0.05). Conclusions: The results indicated that the lentivirus in the nasal mucosa of mice with allergic rhinitis may affect the expression of IL-4, IL-13 and TSLP, and the expression of lentivirus in the nasal mucosa of mice with allergic rhinitis is different from that in the miR-375 lentivirus group. The expression of lentivirus in the nasal mucosa of mice with allergic rhinitis was different.
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DOI：
10.1093/med/9780199683956.003.0028
发表时间：
2015-08
期刊：
Succinct Pediatrics: Evaluation and Management for Common and Critical Care
影响因子：
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作者：
Christine B. Cho;David Goff;Amber M. Patterson
通讯作者：
Christine B. Cho;David Goff;Amber M. Patterson
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DOI：
10.54543/kesans.v2i12.225
发表时间：
2023
期刊：
KESANS : International Journal of Health and Science
影响因子：
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作者：
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DOI：
10.1891/9780826195197.0049
发表时间：
2019-01
期刊：
Canadian Family Practice Guidelines
影响因子：
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作者：
Jill C. Cash;Kathleen Bradbury-Golas
通讯作者：
Jill C. Cash;Kathleen Bradbury-Golas
影响因子：
81.5
作者：
Bousquet, Jean;Anto, Josep M.;Toppila-Salmi, Sanna
通讯作者：
Toppila-Salmi, Sanna
DOI：
10.17116/rosrino202028041246
发表时间：
2020
期刊：
Rossiiskaya rinologiya
影响因子：
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作者：
N. Astafieva;A. A. Baranov-A.;E. Vishneva;N. Daikhes;A.V. Zhestkov;N. Ilyina;O. Karneeva;E.P. Karpova;I.A. Kim;A.I. Kryukov;O. Kurbacheva;R.Ya. Meshkova;L. Namazova-Baranova;N. Nenasheva;G.A. Novik;E. V. Nosulya;K. Pavlova;A. N. Pampura;V. M. Svistushkin;L. Selimzyanova;M. Khaitov;R. M. Khaitov
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N. Astafieva;A. A. Baranov-A.;E. Vishneva;N. Daikhes;A.V. Zhestkov;N. Ilyina;O. Karneeva;E.P. Karpova;I.A. Kim;A.I. Kryukov;O. Kurbacheva;R.Ya. Meshkova;L. Namazova-Baranova;N. Nenasheva;G.A. Novik;E. V. Nosulya;K. Pavlova;A. N. Pampura;V. M. Svistushkin;L. Selimzyanova;M. Khaitov;R. M. Khaitov