Hearing sensitivity differs between zebrafish lines used in auditory research.
Hearing sensitivity differs between zebrafish lines used in auditory research.
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DOI：
10.1016/j.heares.2016.09.004
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发表时间：
2016-11
期刊：
影响因子：
2.8
通讯作者：
Coffin, Allison B.
中科院分区：
文献类型：
--
作者：
Monroe, J. David;Manning, Dustin P.;Uribe, Phillip M.;Bhandiwad, Ashwin;Sisneros, Joseph A.;Smith, Michael E.;Coffin, Allison B.
斑马鱼越来越多地用于听觉研究，部分原因是几种表达毛细胞特异性荧光蛋白的转基因品系的发展。然而，它在很大程度上是未知的转基因表达如何影响听觉表型。我们先前观察到成年Brn 3c：mGFP转基因斑马鱼的听觉灵敏度降低，该转基因斑马鱼在感觉毛细胞中表达膜结合的绿色荧光蛋白（GFP）。在这里，我们研究了听觉敏感性的斑马鱼从多个转基因和背景菌株。我们记录了成年动物的听觉诱发电位，并观察到显着更高的听觉阈值在三个线，表达毛细胞特异性GFP。毛细胞密度和听阈之间没有明显的相关性，表明敏感性降低不是由于毛细胞密度的降低。FM 1 -43摄取减少Brn 3c：mGFP鱼，但不是在其他线路，表明机械转导缺陷可能是负责在Brn 3c动物的听觉表型，但替代机制的基础上增加AEP阈值在其他线路。我们发现减少前脉冲抑制（一种测量神经诱发的行为）在幼虫Brn 3c动物，这表明听觉缺陷的发展早在这条线。我们还发现不同背景品系的成年人之间听觉灵敏度存在显着差异，类似于在小鼠听觉功能模型中观察到的品系差异。我们的研究结果表明，研究人员应该谨慎选择合适的斑马鱼转基因或背景株的听觉研究。
Zebrafish are increasingly used in auditory studies, in part due to the development of several transgenic lines that express hair cell-specific fluorescent proteins. However, it is largely unknown how transgene expression influences auditory phenotype. We previously observed reduced auditory sensitivity in adult Brn3c:mGFP transgenic zebrafish, which express membrane-bound green fluorescent protein (GFP) in sensory hair cells. Here, we examine the auditory sensitivity of zebrafish from multiple transgenic and background strains. We recorded auditory evoked potentials in adult animals and observed significantly higher auditory thresholds in three lines that express hair cell-specific GFP. There was no obvious correlation between hair cell density and auditory thresholds, suggesting that reduced sensitivity was not due to a reduction in hair cell density. FM1-43 uptake was reduced in Brn3c:mGFP fish but not in other lines, suggesting that a mechanotransduction defect may be responsible for the auditory phenotype in Brn3c animals, but that alternate mechanisms underlie the increased AEP thresholds in other lines. We found reduced prepulse inhibition (a measure of auditory-evoked behavior) in larval Brn3c animals, suggesting that auditory defects develop early in this line. We also found significant differences in auditory sensitivity between adults of different background strains, akin to strain differences observed in mouse models of auditory function. Our results suggest that researchers should exercise caution when selecting an appropriate zebrafish transgenic or background strain for auditory studies.
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DOI：
10.1007/s10162-002-3022-x
发表时间：
2003-06-01
影响因子：
2.4
作者：
Harris, JA;Cheng, AG;Rubel, EW
通讯作者：
Rubel, EW
DOI：
10.1523/jneurosci.4559-12.2013
发表时间：
2013-04-24
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Esterberg R;Hailey DW;Coffin AB;Raible DW;Rubel EW
通讯作者：
Rubel EW
影响因子：
2.9
作者：
Brignull HR;Raible DW;Stone JS
通讯作者：
Stone JS
EGFP的黑暗面：多泛素化。
影响因子：
3.7
作者：
Baens, Mathijs;Noels, Heidi;Broeckx, Vicky;Hagens, Sofie;Fevery, Sabine;Billiau, An D.;Vankelecom, Hugo;Marynen, Peter
通讯作者：
Marynen, Peter
影响因子：
2.8
作者：
Coffin, Allison B.;Reinhart, Katherine E.;Owens, Kelly N.;Raible, David W.;Rubel, Edwin W.
通讯作者：
Rubel, Edwin W.