Dynamic AFM detection of the oxidation-induced changes in size, stiffness, and stickiness of low-density lipoprotein.
Dynamic AFM detection of the oxidation-induced changes in size, stiffness, and stickiness of low-density lipoprotein.
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DOI：
10.1186/s12951-020-00727-x
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发表时间：
2020-11-12
影响因子：
10.2
通讯作者：
Chen Y
中科院分区：
文献类型：
--
作者：
Wang K;Li Y;Luo C;Chen Y
低密度脂蛋白（LDL）是一种重要的血浆脂蛋白，将脂质转运到外周组织/细胞。LDL的氧化在动脉粥样硬化的形成中起着关键作用，氧化型LDL是动脉粥样硬化的重要危险因素。LDL/oxLDL的生物力学特性与疾病密切相关。然而，迄今为止，氧化诱导的LDL颗粒的大小和生物力学性质（刚度和粘性）的变化较少研究。在这项研究中，通过检测电泳迁移率、丙二醛产生和共轭二烯形成来证实铜诱导的LDL氧化。然后，利用原子力显微镜（AFM）对LDL颗粒氧化前后及氧化过程中的形貌和生物力学进行了测绘，并对颗粒的尺寸和生物力学力进行了测量和定量分析。氧化诱导LDL颗粒的尺寸和刚度（杨氏模量）显着降低，但粘性（粘附力）显着增加。氧化低密度脂蛋白（oxLDL）的较小，较软，较粘的特性部分解释了其促动脉粥样硬化的作用。这些数据表明，LDL氧化可能通过改变LDL颗粒的大小和生物力学特性来加速动脉粥样硬化的形成。这些数据可能为更好地理解LDL/oxLDL和动脉粥样硬化提供重要信息。
Low-density lipoprotein (LDL) is an important plasma lipoprotein transporting lipids to peripheral tissues/cells. The oxidation of LDL plays critical roles in atherogenesis and its oxidized form (oxLDL) is an important risk factor of atherosclerosis. The biomechanical properties of LDL/oxLDL are closely correlated with the disease. To date, however, the oxidation-induced changes in size and biomechanical properties (stiffness and stickiness) of LDL particles are less investigated. In this study, copper-induced LDL oxidation was confirmed by detecting electrophoretic mobility, malondialdehyde production, and conjugated diene formation. Then, the topographical and biomechanical mappings of LDL particles before/after and during oxidation were performed by using atomic force microscopy (AFM) and the size and biomechanical forces of particles were measured and quantitatively analyzed. Oxidation induced a significant decrease in size and stiffness (Young’s modulus) but a significant increase in stickiness (adhesion force) of LDL particles. The smaller, softer, and stickier characteristics of oxidized LDL (oxLDL) partially explains its pro-atherosclerotic role. The data implies that LDL oxidation probably aggravates atherogenesis by changing the size and biomechanical properties of LDL particles. The data may provide important information for a better understanding of LDL/oxLDL and atherosclerosis.
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DOI：
10.1073/pnas.0601744103
发表时间：
2006-04-18
影响因子：
11.1
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