Effects of endothelial progenitor cell-derived microvesicles on hypoxia/reoxygenation-induced endothelial dysfunction and apoptosis.
Effects of endothelial progenitor cell-derived microvesicles on hypoxia/reoxygenation-induced endothelial dysfunction and apoptosis.
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内皮祖细胞来源的微泡对缺氧/复氧诱导的内皮功能障碍和细胞凋亡的影响
DOI：
10.1155/2013/572729
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发表时间：
2013
影响因子：
--
通讯作者：
Chen Y
中科院分区：
文献类型：
--
作者：
Wang J;Chen S;Ma X;Cheng C;Xiao X;Chen J;Liu S;Zhao B;Chen Y
氧化应激诱导的内皮功能障碍在缺血/再灌注损伤中起关键作用。最近的研究表明，内皮祖细胞衍生的微泡（EPC-MV）可以促进内皮细胞（EC）的血管生成。在这里，我们研究了EPC-MVs对人脑微血管内皮细胞（hb-ECs）缺氧/复氧（H/R）损伤的潜在影响。从在血清剥夺（SD）培养基（饥饿应激，sEPC-MV）或含肿瘤坏死因子-α（TNFα）的SD培养基（凋亡应激，aEPC-MV）中培养的EPC制备MV。用1%O_2低氧6小时复氧24小时的方法建立hb-ECs H/R损伤模型。将H/R hb-EC与EPC-MV共培养。结果表明：（1）H/R hb-ECs功能紊乱，细胞凋亡增加，ROS过量产生;（2）两种不同条件下，EPC-MVs携带caspase 3和miR 126的表达差异显著;（3）sEPC-MVs对H/R hb-ECs功能有促进作用，而aEPC-MVs对H/R hb-ECs有抑制作用;（4）sEPC-MVs和aEPC-MVs的不同作用与miR 126和eNOS表达的变化有关，并可被PI 3 K抑制剂阻断。结论sEPCs-MVs和aEPC-MVs通过其携带的与ROS产生和PI 3 K/eNOS/NO通路相关的RNA在hb-EC凋亡和功能障碍中具有不同的功能。
Oxidative stress-induced endothelial dysfunction plays a key role in ischemia/reperfusion injury. Recent evidence indicates that endothelial progenitor cell-derived microvesicles (EPC-MVs) can promote angiogenesis of endothelial cells (ECs). Here, we investigated the potential effects of EPC-MVs on hypoxia/reoxygenation (H/R) injury in human brain microvascular ECs (hb-ECs). MVs were prepared from EPCs cultured in a serum deprivation (SD) medium (starving stress, sEPC-MVs) or SD medium containing tumor necrosis factor-α (TNFα) (apoptotic stress, aEPC-MVs). H/R injury model of hb-ECs was produced by 6 hr hypoxia (1% O2) and 24 hr reoxygenation. The H/R hb-ECs were co-cultured with EPC-MVs. Results showed that (1) H/R hb-ECs were dysfunctional and coupled with increased apoptosis and ROS overproduction; (2) under two different conditions, EPCs displayed remarkable difference in caspase 3 and miR126 expression, which were carried by the corresponsive EPC-MVs; (3) functionally, sEPC-MVs had beneficial effects on H/R hb-ECs, whereas aEPC-MVs had detrimental effects; (4) the diverse effects of sEPC-MVs and aEPC-MVs were associated with the changes in miR126 and eNOS expression and were abolished by PI3K inhibitor. In conclusion, sEPCs-MVs and aEPC-MVs are functionally different on hb-EC apoptosis and dysfunction via their carried RNAs associated with ROS production and PI3K/eNOS/NO pathway.
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影响因子：
11.1
作者：
Cheng, Henry S.;Sivachandran, Nirojini;Lau, Andrew;Boudreau, Emilie;Zhao, Jimmy L.;Baltimore, David;Delgado-Olguin, Paul;Cybulsky, Myron I.;Fish, Jason E.
通讯作者：
Fish, Jason E.
DOI：
10.1073/pnas.1116848108
发表时间：
2011-12-20
影响因子：
11.1
作者：
Berda-Haddad, Yael;Robert, Stephane;Kaplanski, Gilles
通讯作者：
Kaplanski, Gilles
影响因子：
5
作者：
DEBROE, ME;WIEME, RJ;ROELS, F
通讯作者：
ROELS, F
影响因子：
20.3
作者：
Deregibus, Maria Chiara;Cantaluppi, Vincenzo;Camussi, Giovanni
通讯作者：
Camussi, Giovanni
影响因子：
6.1
作者：
Gatti, Stefano;Bruno, Stefania;Camussi, Giovanni
通讯作者：
Camussi, Giovanni