Toxic Effects of Copper on Antioxidative and Metabolic Enzymes of the Marine Gastropod, Onchidium struma
Toxic Effects of Copper on Antioxidative and Metabolic Enzymes of the Marine Gastropod, Onchidium struma
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铜对海洋腹足动物Onchidium struma抗氧化酶和代谢酶的毒性作用
DOI：
10.1007/s00244-009-9290-2
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发表时间：
2009-02
影响因子：
4
通讯作者：
Cheng, Yong-xu
中科院分区：
文献类型：
--
作者：
Zhao, Yun-long;Mao, Qian;Hou, Xue-li;Wang, Qun;Li, Xiao-bo;Cheng, Yong-xu
本研究的目的是评估急性和亚致死毒性的铜（Cu（2+））的海洋腹足类，文蛤，并检查的实用性的酶参数作为指标的Cu（2+）暴露。在半静态更新试验中，Cu（2+）对O.甲状腺肿为74.80 mg/L，高于其它潮间带生物。在1.35 ~ 4.20 mg/L Cu（2+）染毒1周后，测定肝胰脏和肌肉中Cu/Zn-SOD和CAT的活性，以及酸性磷酸酶（ACP）、碱性磷酸酶（AKP）、谷草转氨酶（GOT）和谷丙转氨酶（GPT）的活性。肝胰脏中Cu/Zn-SOD和CAT活性均高于肌肉。Cu（2+）浓度与肝胰脏Cu/Zn-SOD活性呈负相关，与CAT活性呈正相关。ACP和AKP活性的浓度依赖性变化在肝胰腺中表现出类似的趋势，先增加后减少，最后略有增加。相反，ACP活性与Cu（2+）在测试的浓度范围内呈正相关。在肝胰腺和肌肉中，低浓度Cu（2+）对GOT和GPT都有激活作用，而高浓度Cu（2+）对GOT和GPT都有抑制作用。
The goals of this study were to evaluate the acute and sublethal toxicity of copper (Cu(2+)) on the marine gastropod, Onchidium struma, and to examine the utility of enzymatic parameters as indicators of Cu(2+) exposure. In a semistatic renewal test, the 96-hour median lethal concentration of Cu(2+) for O. struma, 74.80 mg/L, was higher than that for other intertidal species. The activities of the antioxidative enzymes, Cu/Zn superoxide dismutase (Cu/Zn-SOD) and catalase (CAT), and those of the metabolic enzymes-acid phosphatase (ACP), alkaline phosphatase (AKP), glutamic-oxaloacetic transaminase (GOT), and glutamic-pyruvic transaminase (GPT) -in both hepatopancreas and muscle were determined after a 1-week exposure to Cu(2+) (range 1.35 to 4.20 mg/L). The activities of both Cu/Zn-SOD and CAT were higher in hepatopancreas than muscle. In addition, there was a negative correlation between Cu(2+) concentration and Cu/Zn-SOD activity in hepatopancreas, whereas a positive correlation was observed for CAT activity. Concentration-dependent changes in ACP and AKP activity showed a similar trend in hepatopancreas, increasing then decreasing and, finally, a slight increase. In contrast, ACP activity was positively correlated with Cu(2+) across the concentration range tested. In both hepatopancreas and muscle, both GOT and GPT were activated by lower concentrations of Cu(2+) and inhibited at higher concentrations.
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影响因子：
2.6
作者：
M. M. Hanumante-M.;U. D. Deshpande;R. Nagabhushanam
通讯作者：
M. M. Hanumante-M.;U. D. Deshpande;R. Nagabhushanam
影响因子：
2.4
作者：
C. Lim;W. P. Low;S. Chew;Y. Ip
通讯作者：
C. Lim;W. P. Low;S. Chew;Y. Ip
DOI：
10.1007/s00128-003-8853-6
发表时间：
2003-09
影响因子：
2.7
作者：
K. Lehtonen;S. Leiniö
通讯作者：
K. Lehtonen;S. Leiniö
影响因子：
2.6
作者：
U. D. Deshpande;R. Nagabhushanam;M. M. Hanumante-M.
通讯作者：
U. D. Deshpande;R. Nagabhushanam;M. M. Hanumante-M.
影响因子：
11.2
作者：
O. Press;K. DeSantes;S. K. Anderson;F. Geissler
通讯作者：
O. Press;K. DeSantes;S. K. Anderson;F. Geissler