The Protective Effect of New Compound XH-103 on Radiation-Induced GI Syndrome.
The Protective Effect of New Compound XH-103 on Radiation-Induced GI Syndrome.
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新化合物XH-103对辐射引起的胃肠道综合症的保护作用
DOI：
10.1155/2018/3920147
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发表时间：
2018
影响因子：
--
通讯作者：
Tian H
中科院分区：
文献类型：
--
作者：
Dong Y;Cheng Y;Hou Q;Wu J;Li D;Tian H
背景放射性肠损伤是放射治疗的副作用之一。本研究旨在探讨XH-103对放射性小肠损伤的保护作用及其机制。方法C57 BL/6 N小鼠经放射线照射后，给予XH-103治疗。首先，观察了9.0戈伊和11.0戈伊全身照射（TBI）小鼠的存活率。于缺血再灌注后3.5d，取小肠行HE染色。免疫组化法检测肠腺细胞数量、绒毛高度、Ki 67和Lgr 5表达及肠腺细胞凋亡情况。WB法检测p53和Bax蛋白的表达。结果与照射组相比，XH-103能提高小鼠存活率，保护小鼠小肠形态，降低小肠隐窝细胞凋亡率，维持细胞再生，促进隐窝增殖和分化。与IR组相比，XH-103还降低了小肠中p53和Bax的表达。结论XH-103对放射性肠损伤有一定的保护作用，对放射性肠损伤有一定的防护作用。
Background Radiation-induced intestinal injury is one of the side effects in patients receiving radiotherapy. The aim of the present study was to investigate the protective effect of XH-103 on radiation-induced small intestinal injury and to explore its mechanism. Methods C57BL/6N mice were irradiated and treated with XH-103. Firstly, the survival rate of mice exposed to 9.0 Gy and 11.0 Gy total body irradiation (TBI) was examined. Subsequently, at 3.5 d after IR, the small intestinal morphological changes were examined by HE. The numbers of crypt cells, the villus height, the expression of Ki67 and Lgr5, and the apoptotic cells in the intestinal crypts were examined by immunohistochemistry. Furthermore, the expression of p53 and Bax was analyzed by WB. Results Compared to the irradiation group, XH-103 improved the mice survival rate, protected the intestinal morphology of mice, decreased the apoptotic rate of intestinal crypt cells, maintained cell regeneration, and promoted crypt proliferation and differentiation. XH-103 also reduced the expression of p53 and Bax in the small intestine compared to the IR group. Conclusion These data demonstrate that XH-103 can prevent radiation-induced intestinal injury, which is beneficial for the protection of radiation injuries.
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7.2
作者：
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