Changes in CEBPB expression in circulating leukocytes following eccentric elbow-flexion exercise.
Changes in CEBPB expression in circulating leukocytes following eccentric elbow-flexion exercise.
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DOI：
10.1007/s12576-014-0350-7
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发表时间：
2015-01
影响因子：
2.3
通讯作者：
Melzer, David
中科院分区：
文献类型：
--
作者：
Blackwell, Jamie;Harries, Lorna W.;Pilling, Luke C.;Ferrucci, Luigi;Jones, Andrew;Melzer, David
在小鼠模型中，CCAAT增强子结合蛋白β(CEBPB)是M2巨噬细胞介导的肌肉损伤后再生所必需的。在人类中，血液中CEBPB的表达与肌肉力量密切相关。在这项研究中，我们的目的是测试人们血液中CEBPB的表达是否在运动后2天增加，以诱导肌肉损伤和随后的修复。16名健康男性志愿者接受了旨在诱导急性肌肉微损伤的肘屈肌练习。分别于运动前、运动后第1、2、4、7天采集外周血标本。采用定量逆转录-聚合酶链式反应(qRT-PCR)分析CEBPB及其相关基因的表达。肌肉损伤的程度通过最大自愿等长扭矩的下降和血浆肌酸激酶活性来确定。有9名受试者的肌酸激酶活性峰值(第4天)超过10,000 U/L。在这一亚组中，CEBPB的表达在运动后从基线上升到2天(配对样本t(1，8)x=33.72，p=0.006)。运动后相关表达和所选细胞因子变化均未达到显著水平。在人类肌肉损伤运动之后，可以诱导外周血中CEBPB转录本的表达。血液中CEBPB的表达与肌肉力量之间的关联可能与CEBPB依赖的肌肉修复过程一致。本文的在线版本(doi：10.1007/s12576-0140350-7)包含补充材料，授权用户可以使用。
In mouse models, CCAAT enhancer-binding protein beta (CEBPB) is necessary for M2 macrophage-mediated regeneration after muscle injury. In humans, CEBPB expression in blood was strongly associated with muscle strength. In this study we aimed to test whether CEBPB expression in blood in people is increased 2 days after exercise designed to induce muscle damage and subsequent repair. Sixteen healthy male volunteers undertook elbow flexor exercises designed to induce acute muscle micro-damage. Peripheral blood samples were collected at baseline and days 1, 2, 4 and 7 following exercise. Expression of CEBPB and related genes were analysed by qRT-PCR. Extent of muscle damage was determined by decline in maximal voluntary isometric torque and by plasma creatine kinase activity. Nine subjects had peak (day 4) creatine kinase activity exceeding 10,000 U/l. In this subgroup, CEBPB expression was elevated from baseline to 2 days post exercise (paired-samples t (1,8) = 3.72, p = 0.006). Related expression and selected cytokine changes after exercise did not reach significance. Muscle-damaging exercise in humans can be followed by induction of CEBPB transcript expression in peripheral blood. Associations between CEBPB expression in blood and muscle strength may be consistent with the CEBPB-dependent muscle repair process. The online version of this article (doi:10.1007/s12576-014-0350-7) contains supplementary material, which is available to authorized users.
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DOI：
10.1093/gerona/57.5.m326
发表时间：
2002-05-01
影响因子：
5.1
作者：
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通讯作者：
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DOI：
10.1073/pnas.0908641106
发表时间：
2009-10-13
影响因子：
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作者：
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通讯作者：
Nerlov, Claus
影响因子：
7.8
作者：
Harries LW;Pilling LC;Hernandez LD;Bradley-Smith R;Henley W;Singleton AB;Guralnik JM;Bandinelli S;Ferrucci L;Melzer D
通讯作者：
Melzer D