Alkbh4 and Atrn Act Maternally to Regulate Zebrafish Epiboly.
Alkbh4 and Atrn Act Maternally to Regulate Zebrafish Epiboly.
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Alkbh4 和 Atrn 通过母体作用调节斑马鱼 Epiboly
DOI：
10.7150/ijbs.19203
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发表时间：
2017
影响因子：
9.2
通讯作者：
Jia S
中科院分区：
文献类型：
--
作者：
Sun Q;Liu X;Gong B;Wu D;Meng A;Jia S
在胚胎原肠胚形成期间，协调的细胞运动发生，将细胞带到正确的位置。其中，外包产生的第一个明显的形态变化，这是必不可少的早期发育的斑马鱼。尽管其基本的重要性，很少有人知道了解潜在的分子机制。通过使用CRISPR/Cas9技术和使用吗啉代敲低策略产生母体突变系，我们表明母体Alkbh 4缺失导致斑马鱼中严重的外生缺陷。免疫荧光分析表明，Alkbh 4通过调节肌动球蛋白收缩环的形成促进斑马鱼胚胎发育。肌动球蛋白收缩环由肌动蛋白和非肌性肌球蛋白II（NMII）组成。为了进一步研究这一过程，进行酵母双杂交测定，并将Atrn鉴定为Alkbh 4的结合伴侣。结合Alkbh 4的功能结果，我们发现母体Atrn通过调节肌动球蛋白的形成在斑马鱼胚胎形态发生中发挥类似的作用。在分子水平上，我们的数据显示，Atrn倾向于与Alkbh 4的活性形式相互作用，并与其一起调节肌动蛋白的去甲基化，肌动球蛋白的形成，以及随后的胚胎发育。
During embryonic gastrulation, coordinated cell movements occur to bring cells to their correct position. Among them, epiboly produces the first distinct morphological changes, which is essential for the early development of zebrafish. Despite its fundamental importance, little is known to understand the underlying molecular mechanisms. By generating maternal mutant lines with CRISPR/Cas9 technology and using morpholino knockdown strategy, we showed that maternal Alkbh4 depletion leads to severe epiboly defects in zebrafish. Immunofluorescence assays revealed that Alkbh4 promotes zebrafish embryonic epiboly through regulating actomyosin contractile ring formation, which is composed of Actin and non-muscular myosin II (NMII). To further investigate this process, yeast two hybridization assay was performed and Atrn was identified as a binding partner of Alkbh4. Combining with the functional results of Alkbh4, we found that maternal Atrn plays a similar role in zebrafish embryonic morphogenesis by regulating actomyosin formation. On the molecular level, our data revealed that Atrn prefers to interact with the active form of Alkbh4 and functions together with it to regulate the demethylation of Actin, the actomyosin formation, and subsequently the embryonic epiboly.
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影响因子：
4.3
作者：
Hida T;Wakamatsu K;Sviderskaya EV;Donkin AJ;Montoliu L;Lynn Lamoreux M;Yu B;Millhauser GL;Ito S;Barsh GS;Jimbow K;Bennett DC
通讯作者：
Bennett DC
ALKBH4 依赖性肌动蛋白去甲基化调节肌动球蛋白动力学
影响因子：
16.6
作者：
通讯作者：
--
影响因子：
3.7
作者：
Nilsen A;Fusser M;Greggains G;Fedorcsak P;Klungland A
通讯作者：
Klungland A
影响因子：
2.5
作者：
Cheng, JC;Miller, AL;Webb, SE
通讯作者：
Webb, SE
影响因子：
30.8
作者：
He, L;Gunn, TM;Barsh, GS
通讯作者：
Barsh, GS