Histology and molecular pathology of iliotibial tract contracture in patients with gluteal muscle contracture
Histology and molecular pathology of iliotibial tract contracture in patients with gluteal muscle contracture
复制标题
臀肌挛缩症患者髂胫束挛缩的组织学和分子病理学
DOI：
10.1042/bsr20181351
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发表时间：
2019-08
影响因子：
4
通讯作者：
刘玉杰
中科院分区：
文献类型：
--
作者：
袁邦拓;曲峰;王少霞;齐玮;申学振;李春宝;刘玉杰
本研究旨在探讨髂胫束的病理变化并探讨其与臀肌挛缩的关系。从 6 名在手术治疗期间出现臀肌和髂胫束挛缩的患者中采集了挛缩性髂胫束样本。采集 6 名接受股骨头缺血性坏死手术且无臀肌和髂胫束挛缩的患者的正常髂胫束样本。使用苏木精和伊红 (H&E)、马森三色和天狼星红对组织样本进行染色。使用定量实时PCR和蛋白质印迹法测定各种组织修复基因的mRNA和蛋白质水平。正常和收缩性髂胫束均由 I 型和 III 型胶原组成。与正常髂胫束相比，收缩性髂胫束的 III 型胶原比例明显更高。与正常髂胫束相比，收缩性髂胫束中组织修复基因TGFβ1、bFGF和基质金属蛋白酶-1（MMP-1）的mRNA表达水平和蛋白水平上调。然而，与正常髂胫束相比，收缩性髂胫束中金属蛋白酶组织抑制剂-1（TIMP）的mRNA表达水平和蛋白水平下调。臀肌和髂胫束的挛缩具有相似的组织学和分子病理学。我们的结果表明，髂胫束挛缩继发于臀肌挛缩，并且是一个持续的组织修复过程。
The present study aimed to examine the pathologic changes of the iliotibial tract and discusses its relationship with gluteal muscle contracture. Samples of contractual iliotibial tracts were collected from six patients with contractures of the gluteal muscles and iliotibial tracts during their surgical treatment. Samples of normal iliotibial tracts were collected from six patients receiving surgeries for avascular necrosis of the femoral head who had no contractures of the gluteal muscles and iliotibial tracts. The tissue samples were stained using Hematoxylin and Eosin (H&E), Masson’s trichrome, and Sirius Red. The mRNA and protein levels of various tissue repair genes were determined using quantitative real-time PCR and Western blotting. Both the normal and contractual iliotibial tracts consisted of type I and III collagens. The contractual iliotibial tracts had a significantly higher proportion of type III collagen in comparison with the normal iliotibial tracts. The mRNA expression levels and protein levels of tissue repair genes TGFβ 1, bFGF, and matrix metalloproteinase-1 (MMP-1) in the contractual iliotibial tracts were up-regulated in comparison with that in the normal iliotibial tracts. However, the mRNA expression levels and protein levels of tissue inhibitors of metalloproteinase-1 (TIMP) in the contractual iliotibial tracts were down-regulated in comparison with that in the normal iliotibial tracts. The contractures of both the gluteal muscles and the iliotibial tracts share similar histology and molecular pathology. Our results indicate that iliotibial tract contracture is secondary to the gluteal muscle contracture and is a constant tissue repair process.
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DOI：
10.1111/j.1346-8138.1992.tb03751.x
发表时间：
1992-11
期刊：
The Journal of Dermatology
影响因子：
--
作者：
R. Tsuboi;C. Sato;C. Shi;H. Ogawa
通讯作者：
R. Tsuboi;C. Sato;C. Shi;H. Ogawa
DOI：
10.1302/0301-620x.54b3.532
发表时间：
1972-08
期刊：
The Journal of bone and joint surgery. British volume
影响因子：
--
作者：
M. Mehta
通讯作者：
M. Mehta
DOI：
10.1302/0301-620x.50b2.278
发表时间：
1968
期刊：
The Journal of bone and joint surgery. British volume
影响因子：
--
作者：
P. Williams
通讯作者：
P. Williams
影响因子：
2.7
作者：
Al Bayati, Mohammed Ali;Kraidy, Bakir Kadhum
通讯作者：
Kraidy, Bakir Kadhum
影响因子：
2.7
作者：
DREHOBL, P
通讯作者：
DREHOBL, P