Genome-Wide Characterization of R2R3-MYB Transcription Factors in Pitaya Reveals a R2R3-MYB Repressor HuMYB1 Involved in Fruit Ripening through Regulation of Betalain Biosynthesis by Repressing Betalain Biosynthesis-Related Genes.
Genome-Wide Characterization of R2R3-MYB Transcription Factors in Pitaya Reveals a R2R3-MYB Repressor HuMYB1 Involved in Fruit Ripening through Regulation of Betalain Biosynthesis by Repressing Betalain Biosynthesis-Related Genes.
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作者：
Xie F;Hua Q;Chen C;Zhang Z;Zhang R;Zhao J;Hu G;Chen J;Qin Y
MYB （myeloblastosis）超家族是植物中最丰富的转录因子（tf）之一，可调节多种生物过程。然而，MYB TFs在火龙果中的分子特征和功能尚不清楚。迄今为止，尚未在仙人掌科物种中进行该基因家族的全基因组特征分析。本研究从黄颡鱼基因组数据中鉴定出105个R2R3-MYB成员，并对其保守基序、生理生化特征、染色体位置、共系关系、基因结构和系统发育进行了分析。表达分析表明，3个上调的HuMYBs和22个下调的HuMYBs可能参与了火龙果的成熟过程。对R2R3-MYB阻遏物的系统发育分析表明，在含有R2R3-MYB阻遏物的支系中存在7个HuMYBs （HuMYB1、HuMYB21、HuMYB48、HuMYB49、HuMYB72、HuMYB78和HuMYB101）。随着“观花红”火龙果果实成熟过程中β素的积累，HuMYB1和HuMYB21显著下调。然而，只有HuMYB1具有R2和R3重复的C1、C2、C3和C4基序。HuMYB1仅定位于细胞核，并表现出转录抑制能力。双荧光素酶报告基因实验表明，HuMYB1抑制甜菜素相关基因：HuADH1、huyp76ad1 -1和HuDODA1的表达。这些结果表明，HuMYB1是火龙果成熟过程中甜菜素生物合成的潜在抑制因子。我们的研究结果首次对参与火龙果甜菜素生物合成的R2R3-MYB亚家族进行了全基因组分析，并将为未来对该基因家族的功能分析提供便利。
The MYB (myeloblastosis) superfamily constitutes one of the most abundant transcription factors (TFs) regulating various biological processes in plants. However, the molecular characteristics and functions of MYB TFs in pitaya remain unclear. To date, no genome-wide characterization analysis of this gene family has been conducted in the Cactaceae species. In this study, 105 R2R3-MYB members were identified from the genome data of Hylocereus undatus and their conserved motifs, physiological and biochemical characteristics, chromosome locations, synteny relationship, gene structure and phylogeny were further analyzed. Expression analyses suggested that three up-regulated HuMYBs and twenty-two down-regulated HuMYBs were probably involved in fruit ripening of pitaya. Phylogenetic analyses of R2R3-MYB repressors showed that seven HuMYBs (HuMYB1, HuMYB21, HuMYB48, HuMYB49, HuMYB72, HuMYB78 and HuMYB101) were in clades containing R2R3-MYB repressors. HuMYB1 and HuMYB21 were significantly down-regulated with the betalain accumulation during fruit ripening of ‘Guanhuahong’ pitaya (H. monacanthus). However, only HuMYB1 had R2 and R3 repeats with C1, C2, C3 and C4 motifs. HuMYB1 was localized exclusively to the nucleus and exhibited transcriptional inhibition capacities. Dual luciferase reporter assay demonstrated that HuMYB1 inhibited the expression of betalain-related genes: HuADH1, HuCYP76AD1-1 and HuDODA1. These results suggested that HuMYB1 is a potential repressor of betalain biosynthesis during pitaya fruit ripening. Our results provide the first genome-wide analyses of the R2R3-MYB subfamily involved in pitaya betalain biosynthesis and will facilitate functional analysis of this gene family in the future.
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DOI：
10.1111/nph.13441
发表时间：
2015-09
期刊：
The New phytologist
影响因子：
--
作者：
Brockington SF;Yang Y;Gandia-Herrero F;Covshoff S;Hibberd JM;Sage RF;Wong GK;Moore MJ;Smith SA
通讯作者：
Smith SA
苹果bZIP转录因子MdbZIP44调节脱落酸促进花青素积累
影响因子：
7.3
作者：
An, Jian-Ping;Yao, Ji-Fang;Hao, Yu-Jin
通讯作者：
Hao, Yu-Jin
影响因子：
6.5
作者：
Hua, Qingzhu;Chen, Canbin;Qin, Yonghua
通讯作者：
Qin, Yonghua
影响因子：
4.6
作者：
He Q;Jones DC;Li W;Xie F;Ma J;Sun R;Wang Q;Zhu S;Zhang B
通讯作者：
Zhang B
影响因子：
8.7
作者：
Chen JY;Xie FF;Cui YZ;Chen CB;Lu WJ;Hu XD;Hua QZ;Zhao J;Wu ZJ;Gao D;Zhang ZK;Jiang WK;Sun QM;Hu GB;Qin YH
通讯作者：
Qin YH