Selective cellular imaging with lanthanide-based upconversion nanoparticles.
Selective cellular imaging with lanthanide-based upconversion nanoparticles.
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DOI：
10.1002/jbio.201800256
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发表时间：
2019-04
影响因子：
2.8
通讯作者：
Saha S
中科院分区：
文献类型：
--
作者：
Nampi PP;Vakurov A;Mackenzie LE;Scrutton NS;Millner PA;Jose G;Saha S
通过水热途径合成了具有氟化钇钠、掺杂有Yb 3+（敏化剂）和Er 3+（活化剂）的NaYF 4（主体晶格）的上转换纳米颗粒（UCNP），其结合聚乙烯亚胺（PEI）以用于其在水中的长期稳定性。直径为20 ± 4 nm的阳离子PEI改性的UCNP显示出+36.5 mV的ζ电位值，并在976 nm激光激发下显示出强烈的可见红色发光和低强度绿色发射。经3-（4，5-二甲基噻唑-2基）-2，5-二苯基溴化四氮唑（MTT）试验证明，在广泛的UCNP浓度范围（0.3 ng/mL-0.3 mg/mL）内，颗粒对内皮细胞无毒，显示90%至100%的细胞活力，用于多光子成像。这里报道的多光子细胞成像和发射光谱数据证明分散在细胞培养基中的UCNP主要集中在细胞质中而不是细胞核中。在生物系统中，从PEI涂覆的UCNP到周围介质的红色发光的能量转移也通过光谱测量突出显示。因此，本研究的结果表明，UCNP可以与对细胞核具有选择性的多光子染料（例如，4′，6-diamidino-2-phenylindole（DAPI））一起用于细胞质选择性成像。水热法合成的高荧光水分散性聚乙烯亚胺包覆的NaYF 4/Yb 3 +/Er 3+上转换纳米粒子是一种很有前途的用于细胞质成像的无毒生物相容性材料。
Upconversion nanoparticles (UCNPs) with sodium yttrium fluoride, NaYF4 (host lattice) doped with Yb3+ (sensitizer) and Er3+ (activator) were synthesized via hydrothermal route incorporating polyethyleneimine (PEI) for their long‐term stability in water. The cationic PEI‐modified UCNPs with diameter 20 ± 4 nm showed a zeta potential value of +36.5 mV and showed an intense, visible red luminescence and low‐intensity green emission with 976 nm laser excitation. The particles proven to be nontoxic to endothelial cells, with a 3‐(4,5‐dimethylthiazol‐2yl)‐2,5‐diphenyltetrazolium bromide (MTT) assay, showing 90% to 100% cell viability, across a wide range of UCNP concentrations (0.3 ng/mL‐0.3 mg/mL) were used in multiphoton imaging. Multiphoton cellular imaging and emission spectroscopy data reported here prove that the UCNPs dispersed in cell culture media are predominantly concentrated in the cytoplasm than the cell nucleus. The energy transfer from PEI‐coated UCNPs to surrounding media for red luminescence in the biological system is also highlighted with spectroscopic measurements. Results of this study propose that UCNPs can, therefore, be used for cytoplasm selective imaging together with multiphoton dyes (eg, 4′,6‐diamidino‐2‐phenylindole (DAPI)) that are selective to cell nucleus. Highly fluorescent water‐dispersible polyethyleneimine‐coated NaYF4/Yb3+/Er3+ upconversion nanoparticles synthesized using hydrothermal method are promising nontoxic biocompatible materials for imaging cellular cytoplasm.
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Nayf的理论分子量（4）：重新转换纳米颗粒。
影响因子：
4.6
作者：
Mackenzie LE;Goode JA;Vakurov A;Nampi PP;Saha S;Jose G;Millner PA
通讯作者：
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影响因子：
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影响因子：
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