The CLS2 gene encodes a protein with multiple membrane-spanning domains that is important Ca2+ tolerance in yeast
The CLS2 gene encodes a protein with multiple membrane-spanning domains that is important Ca2+ tolerance in yeast
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CLS2 基因编码具有多个跨膜结构域的蛋白质，该蛋白质对于酵母的 Ca2 耐受性至关重要
DOI：
10.1007/bf00288599
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Y. Anraku
中科院分区：
文献类型：
--
作者：
Yoko Takita;Y. Ohya;Y. Anraku
遗传筛选的酿酒酵母突变体缺陷的Ca 2+稳态确定cls2，它表现出一个特定的Ca 2+敏感的生长表型。我们在这里描述了CLS2基因和CLS2突变的多拷贝抑制基因（命名为BCL 21，用于绕过CLS2）。CLS2基因编码410个氨基酸残基的多肽，其亲水特性表明预测的Cls2蛋白（Cls2p）含有10个推定的跨膜区。表达表位标记的Cls2p的酵母细胞的免疫荧光染色表明Cls2p定位于内质网（ER）膜。cls2破坏菌株是有活力的，但显示出与原始cls2突变体一样的Ca 2+敏感表型。BCL 21抑制cls2破坏突变，表明多拷贝抑制不需要Cls2p。即使在引入携带BCL 21的复制质粒后也观察到cls2的抑制。BCL 21基因编码382个氨基酸残基的蛋白质，并且与SUR1基因相同。Sur1最初是作为RVS 161的抑制因子分离的，RVS 161在营养物饥饿条件下具有降低的生存力。多拷贝抑制的可能机制进行了讨论。
Genetic screening of Saccharomyces cerevisiae mutants defective in Ca2+ homeostasis identified cls2, which exhibits a specific Ca2+-sensitive growth phenotype. We describe here the CLS2 gene and a multicopy suppressor (named BCL21, for bypass of CLS2) of the cls2 mutation. The CLS2 gene encodes a polypeptide of 410 amino acid residues, and its hydropathy profile indicates that the predicted Cls2 protein (Cls2p) contains ten putative membrane spanning regions. Immunofluorescent staining of the yeast cells expressing epitopetagged Cls2p suggests that Cls2p is localized to endoplasmatic reticulum (ER) membrane. A cls2 disruption strain is viable, but shows a Ca2+-sensitive phenotype like the original cls2 mutants. BCL21 suppresses the cls2 disruption mutation, indicating that the multicopy suppression does not require the Cls2p. Suppression of cls2 was observed even after introduction of a singlecopy plasmid harboring BCL21. The BCL21 gene encodes a protein of 382 amino acid residues and is identical to the SUR1 gene. sur1 was originally isolated as a suppressor of rvs161, which has reduced viability in nutrient starvation conditions. Possible mechanisms of the multicopy suppression are discussed.
影响因子：
--
作者：
J. Pringle;R. Preston;A. Adams;T. Stearns;D. Drubin;B. Haarer;E. Jones
通讯作者：
J. Pringle;R. Preston;A. Adams;T. Stearns;D. Drubin;B. Haarer;E. Jones
DOI：
10.1073/pnas.86.15.5786
发表时间：
1989-08-01
影响因子：
11.1
作者：
HARTMANN, E;RAPOPORT, TA;LODISH, HF
通讯作者：
LODISH, HF
影响因子：
3.3
作者：
ANTEBI, A;FINK, GR
通讯作者：
FINK, GR