Efflux inhibitor suppresses Streptococcus mutans virulence properties
Efflux inhibitor suppresses Streptococcus mutans virulence properties
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外排抑制剂抑制变形链球菌的毒力特性
DOI：
10.1093/femsle/fnx033
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发表时间：
2017-04
影响因子：
2.1
通讯作者：
Ling JQ
中科院分区：
文献类型：
--
作者：
Zeng Huihui;Liu Jia;Ling Junqi;Zeng Huihui;Ling JQ
摘要外排泵在细菌致病性中发挥着重要作用，外排抑制剂（EI）已被证明可以有效抑制细菌的毒力特性。然而，关于变形链球菌的EI知之甚少，变形链球菌是一种众所周知的龋齿诱导细菌。在这项研究中，我们确定了S的EI。通过溴化乙锭外排试验研究EI对变形链球菌的影响。变形菌的致龋性和应激反应的毒力。结果表明，利血平抑制S.变形杆菌，以及改变的生物膜结构和胞外多糖分布。利血平暴露后，糖基转移酶活性也受到抑制。来自定量真实的时间PCR的数据表明，利血平显著改变了群体感应和毒力相关基因的表达谱。这些发现表明利血平是一种很有前途的辅助抗龋剂，因为它抑制了S.变种人
Abstract It is well established that efflux pumps play important roles in bacterial pathogenicity and efflux inhibitors (EIs) have been proved to be effective in suppressing bacterial virulence properties. However, little is known regarding the EI of Streptococcus mutans, a well‐known caries‐inducing bacterium. In this study, we identified the EI of S. mutans through ethidium bromide efflux assay and investigated how EI affected S. mutans virulence regarding the cariogenicity and stress response. Results indicated that reserpine, the identified EI, suppressed acid tolerance, mutacin production and transformation efficiency of S. mutans, and modified biofilm architecture and extracellular polysaccharide distribution. Suppressed glycosyltransferase activity was also noted after reserpine exposure. The data from quantitative real‐time‐PCR demonstrated that reserpine significantly altered the expression profile of quorum‐sensing and virulence‐associated genes. These findings suggest that reserpine represents a promising adjunct anticariogenic agent in that it suppresses virulence properties of S. mutans.
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影响因子：
6.7
作者：
Xiao J;Klein MI;Falsetta ML;Lu B;Delahunty CM;Yates JR 3rd;Heydorn A;Koo H
通讯作者：
Koo H
DOI：
--
发表时间：
2011-05
期刊：
The Indian Journal of Medical Research
影响因子：
--
作者：
Mradula Singh;G. Jadaun;Ramdas;K. Srivastava;Vipin Chauhan;Ritu Mishra;K. Gupta;S. Nair;D. Chauhan;V.D. Sharma;K. Venkatesan;V. Katoch
通讯作者：
Mradula Singh;G. Jadaun;Ramdas;K. Srivastava;Vipin Chauhan;Ritu Mishra;K. Gupta;S. Nair;D. Chauhan;V.D. Sharma;K. Venkatesan;V. Katoch
影响因子：
4.9
作者：
Xu, Xin;Zhou, Xue D.;Wu, Christine D.
通讯作者：
Wu, Christine D.
影响因子：
4.4
作者：
Kvist, Malin;Hancock, Viktoria;Klemm, Per
通讯作者：
Klemm, Per
影响因子：
4.4
作者：
Zachary D. Moye;Minjun Son;Ariana E Rosa-Alberty;L. Zeng;Sang-Joon Ahn;S. Hagen;R. Burne
通讯作者：
Zachary D. Moye;Minjun Son;Ariana E Rosa-Alberty;L. Zeng;Sang-Joon Ahn;S. Hagen;R. Burne