Phage shock proteins B and C prevent lethal cytoplasmic membrane permeability in Yersinia enterocolitica.
Phage shock proteins B and C prevent lethal cytoplasmic membrane permeability in Yersinia enterocolitica.
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DOI：
10.1111/j.1365-2958.2012.08120.x
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发表时间：
2012-08
影响因子：
3.6
通讯作者：
Darwin AJ
中科院分区：
文献类型：
--
作者：
Horstman NK;Darwin AJ
细菌噬菌体休克蛋白（Psp）应激反应系统是由影响细胞质膜的事件激活的。因此，Psp蛋白水平升高，包括PspA，它被认为是胁迫耐受性的主要效应因子。小肠结肠炎耶尔森菌和Psp系统缺陷的相关细菌对造孔分泌素蛋白的错误定位高度敏感。然而，为什么分泌素对psp无效菌株有毒性，而其他一些psp诱导剂没有毒性，还没有解释。此外，先前的工作已经导致混淆和有争议的建议，即PspA不参与减轻分泌素毒性。在这里，我们已经建立了分泌素毒性的量在psp零菌株和细胞质膜对大分子的渗透程度之间的相关性。这导致一种形态变化，类似于细胞的质解。此外，使用具有失调Psp蛋白的新菌株使我们能够获得明确的证据，证明PspA不是分泌素应激耐受性所必需的。总之，我们的数据表明分泌素多聚体杀死psp零细胞的机制是通过引起细胞质膜通透性屏障的深刻缺陷。这允许与环境进行致命的分子交换，这是PspB和PspC蛋白可以阻止的。
The bacterial phage shock protein (Psp) stress response system is activated by events affecting the cytoplasmic membrane. In response, Psp protein levels increase, including PspA, which has been implicated as the master effector of stress tolerance. Yersinia enterocolitica and related bacteria with a defective Psp system are highly sensitive to the mislocalization of pore-forming secretin proteins. However, why secretins are toxic to psp null strains, whereas some other Psp-inducers are not, has not been explained. Furthermore, previous work has led to the confounding and disputable suggestion that PspA is not involved in mitigating secretin toxicity. Here we have established a correlation between the amount of secretin toxicity in a psp null strain and the extent of cytoplasmic membrane permeability to large molecules. This leads to a morphological change resembling cells undergoing plasmolysis. Furthermore, using novel strains with dis-regulated Psp proteins has allowed us to obtain unequivocal evidence that PspA is not required for secretin-stress tolerance. Together, our data suggest that the mechanism by which secretin multimers kill psp null cells is by causing a profound defect in the cytoplasmic membrane permeability barrier. This allows lethal molecular exchange with the environment, which the PspB and PspC proteins can prevent.
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影响因子：
3.2
作者：
De Rossi, E;Branzoni, M;Ciferri, O
通讯作者：
Ciferri, O
影响因子：
3.2
作者：
Burghout, P;Beckers, F;Koster, M
通讯作者：
Koster, M
影响因子：
3.6
作者：
Gueguen E;Savitzky DC;Darwin AJ
通讯作者：
Darwin AJ
影响因子：
3.2
作者：
CHANG, ACY;COHEN, SN
通讯作者：
COHEN, SN
影响因子：
3.2
作者：
GUZMAN, LM;BELIN, D;BECKWITH, J
通讯作者：
BECKWITH, J