Pyrimidine degradation influences germination seedling growth and production of Arabidopsis seeds.
Pyrimidine degradation influences germination seedling growth and production of Arabidopsis seeds.
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DOI：
10.1093/jxb/err251
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发表时间：
2011-11
影响因子：
6.9
通讯作者：
Möhlmann T
中科院分区：
文献类型：
--
作者：
Cornelius S;Witz S;Rolletschek H;Möhlmann T
PYD 1（二氢嘧啶脱氢酶）启动嘧啶核碱基的降解并位于质体中。在这项研究中，PYD 1采用T-DNA敲除突变体和过表达进行了生理分析。PYD 1基因敲除突变体限制了外源提供的尿嘧啶的降解，并在整个发育过程中在植物器官中积累了高水平的尿嘧啶，特别是在干种子中。此外，PYD 1基因敲除突变体表现出延迟发芽，伴随着低转化酶活性和降低单糖水平。脱落酸（阿坝）是种子萌发的重要调节因子，PYD 1基因敲除突变体中ABA响应基因表达失调。与观察到的增加PYD 1表达在野生型幼苗阿坝处理后，干扰PYD 1与阿坝信号可以假定。组成型PYD 1过表达突变体表现出增加的增长和更高的种子数相比，野生型和敲除突变体植物。在衰老过程中，PYD 1表达增加，以允许尿嘧啶催化。由此得出结论，在发育早期和种子生产期间，需要PYD 1来平衡嘧啶催化剂与补救剂。
PYD1 (dihydropyrimidine dehydogenase) initiates the degradation of pyrimidine nucleobases and is located in plastids. In this study, a physiological analysis of PYD1 employing T-DNA knockout mutants and overexpressors was carried out. PYD1 knockout mutants were restricted in degradation of exogenously provided uracil and accumulated high uracil levels in plant organs throughout development, especially in dry seeds. Moreover, PYD1 knockout mutants showed delayed germination which was accompanied by low invertase activity and decreased monosaccharide levels. Abscisic acid (ABA) is an important regulator of seed germination, and ABA-responsive genes were deregulated in PYD1 knockout mutants. Together with an observed increased PYD1 expression in wild-type seedlings upon ABA treatment, an interference of PYD1 with ABA signalling can be assumed. Constitutive PYD1 overexpression mutants showed increased growth and higher seed number compared with wild-type and knockout mutant plants. During senescence PYD1 expression increased to allow uracil catabolism. From this it is concluded that early in development and during seed production PYD1 is needed to balance pyrimidine catabolism versus salvage.
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影响因子：
7.4
作者：
Scheible, WR;Morcuende, R;Stitt, M
通讯作者：
Stitt, M
DOI：
10.1073/pnas.0900689106
发表时间：
2009-08-04
影响因子：
11.1
作者：
Barratt, D. H. Paul;Derbyshire, Paul;Smith, Alison M.
通讯作者：
Smith, Alison M.
影响因子：
3.3
作者：
Rawls, JM
通讯作者：
Rawls, JM
影响因子：
4.8
作者：
Schmidt, A;Su, YH;Desimone, M
通讯作者：
Desimone, M
影响因子：
7.2
作者：
Clough, SJ;Bent, AF
通讯作者：
Bent, AF