Marine bromophenol bis (2,3-dibromo-4,5-dihydroxy-phenyl)-methane inhibits the proliferation, migration, and invasion of hepatocellular carcinoma cells via modulating β1-integrin/FAK signaling.
Marine bromophenol bis (2,3-dibromo-4,5-dihydroxy-phenyl)-methane inhibits the proliferation, migration, and invasion of hepatocellular carcinoma cells via modulating β1-integrin/FAK signaling.
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海洋溴酚双 (2,3-二溴-4,5-二羟基-苯基)-甲烷通过调节 β1-整合素/FAK 信号传导抑制肝细胞癌细胞的增殖、迁移和侵袭
DOI：
10.3390/md13021010
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发表时间：
2015-02-13
期刊：
影响因子：
5.4
通讯作者：
Shi D
中科院分区：
文献类型：
--
作者：
Wu N;Luo J;Jiang B;Wang L;Wang S;Wang C;Fu C;Li J;Shi D
双（2，3-二溴-4，5-二羟基-苯基）-甲烷（BDDPM）是一种来源于海藻的天然溴酚化合物。已有研究表明BDDPM具有抗菌活性。在本研究中，我们发现BDDPM对广泛的肿瘤细胞具有细胞毒活性，包括BEL-7402细胞（IC 50 = 8.7 μg/mL）。进一步的研究表明，在细胞凋亡开始之前，BDDPM通过减少细胞与细胞外基质（ECM）的粘附来诱导BEL-7402细胞脱离。分离实验表明，用低浓度的BDDPM（5.0 μg/mL）处理BEL-7402细胞显著抑制细胞与纤连蛋白和胶原IV的粘附以及细胞迁移和侵袭。高剂量BDDPM（10.0 μg/mL）可完全抑制BEL-7402细胞的迁移，并显著降低MMP-2和MMP-9的表达水平。BDDPM可下调β1整合素和粘着斑激酶（FAK）的表达。这项研究表明，BDDPM具有潜在的开发作为一种新型的抗癌治疗药物，由于其抗转移活性，也表明，BDDPM，具有独特的化学结构，可以作为一个先导化合物，合理的药物设计和未来的抗癌药物的发展。
Bis (2,3-dibromo-4,5-dihydroxy-phenyl)-methane (BDDPM) is a natural bromophenol compound derived from marine algae. Previous reports have shown that BDDPM possesses antimicrobial activity. In the present study, we found that BDDPM has cytotoxic activity on a wide range of tumor cells, including BEL-7402 cells (IC50 = 8.7 μg/mL). Further studies have shown that prior to the onset of apoptosis, the BDDPM induces BEL-7402 cell detachment by decreasing the adherence of cells to the extracellular matrix (ECM). Detachment experiments have shown that the treatment of BEL-7402 cells with low concentrations of BDDPM (5.0 μg/mL) significantly inhibits cell adhesion to fibronectin and collagen IV as well as cell migration and invasion. High doses of BDDPM (10.0 μg/mL) completely inhibit the migration of BEL-7402 cells, and the expression level of MMPs (MMP-2 and MMP-9) is significantly decreased. Moreover, the expression of β1-integrin and focal adhesion kinase (FAK) is found to be down-regulated by BDDPM. This study suggests that BDDPM has a potential to be developed as a novel anticancer therapeutic agent due to its anti-metastatic activity and also indicates that BDDPM, which has a unique chemical structure, could serve as a lead compound for rational drug design and for future development of anticancer agents.
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影响因子：
5.4
作者：
Liu M;Zhang W;Wei J;Lin X
通讯作者：
Lin X
影响因子：
50.5
作者：
Besse, B.;Tsao, L. C.;Belani, C. P.
通讯作者：
Belani, C. P.
海洋溴苯酚双(2,3-二溴-4,5-二羟基苯甲基)醚在体外诱导 K562 细胞线粒体凋亡并抑制拓扑异构酶 I。
影响因子：
3.5
作者：
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通讯作者：
Lin, Xiukun
影响因子：
9.7
作者：
Ma, Wen-Lung;Jeng, Long-Bin;Chang, Chawnshang
通讯作者：
Chang, Chawnshang
影响因子：
2.9
作者：
Wang, Feng-xia;Wu, Ning;Lin, Xiu-kun
通讯作者：
Lin, Xiu-kun