Serotonin‐containing neurons in basal insects: In search of ground patterns among tetraconata
Serotonin‐containing neurons in basal insects: In search of ground patterns among tetraconata
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基础昆虫中含有血清素的神经元：寻找四角虫中的基础模式
DOI：
10.1002/cne.24043
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发表时间：
2017
影响因子：
2.5
通讯作者：
G Bicker
中科院分区：
文献类型：
--
作者：
Stemme T;Stern M;G Bicker
Tetraconata的腹侧神经索含有相对较少的5-羟色胺免疫反应神经元，这有利于细胞的个体识别及其特有的轴突形态。这提供了在单细胞水平上建立同源性的相当独特的可能性。由于四胞动物内部的系统发育关系仍有争议，对可单独识别的神经元的比较有助于揭开这些问题。5-羟色胺免疫反应性已经在许多四酸盐分类群中被研究，导致了主要谱系的假设的基本模式的重建。然而，对基础昆虫的详细描述仍然缺乏，但对于有意义的进化考虑是至关重要的。本研究观察了恒河猴(Thermobia home tica，Lepisma sucharina，Atelura formicaria)和古猿(Machilis germanica，Dilta Hibernica)腹侧神经索内5-羟色胺免疫反应神经元的形态。为了提高免疫细胞化学分辨率，我们还进行了5-羟基-L-色氨酸和5-羟色胺的预孵育实验。此外，我们还检查了色氨酸羟基酶的免疫标记，这是一种与5-羟色胺合成相关的酶。除了在腹神经索的每个神经节中普遍发现的前外侧、内侧和后外侧神经元组外，我们还发现了其他几个免疫反应细胞，这些细胞似乎在其他四酮类中没有对应的细胞。此外，我们发现并不是所有的免疫反应神经元都产生5-羟色胺，但具有摄取5-羟色胺的能力。与主要四酸类中含5-羟色胺神经元的模式比较表明，雷米足纲、头足纲和六足纲之间存在密切的系统发育关系，支持小甲目的假说。J.Comp.Neurol.，2016。©2016威利期刊公司J.Comp.神经。2017年525：79-115。©2016 Wiley期刊，Inc.
The ventral nerve cord of Tetraconata contains a comparably low number of serotonin‐immunoreactive neurons, facilitating individual identification of cells and their characteristic neurite morphology. This offers the rather unique possibility of establishing homologies at the single cell level. Because phylogenetic relationships within Tetraconata are still discussed controversially, comparisons of individually identifiable neurons can help to unravel these issues. Serotonin immunoreactivity has been investigated in numerous tetraconate taxa, leading to reconstructions of hypothetical ground patterns for major lineages. However, detailed descriptions of basal insects are still missing, but are crucial for meaningful evolutionary considerations. We investigated the morphology of individually identifiable serotonin‐immunoreactive neurons in the ventral nerve cord of Zygentoma (Thermobia domestica, Lepisma saccharina, Atelura formicaria) and Archaeognatha (Machilis germanica, Dilta hibernica). To improve immunocytochemical resolution, we also performed preincubation experiments with 5‐hydroxy‐L‐tryptophan and serotonin. Additionally, we checked for immunolabeling of tryptophan hydroxylase, an enzyme associated with the synthesis of serotonin. Besides the generally identified groups of anterolateral, medial, and posterolateral neurons within each ganglion of the ventral nerve cord, we identified several other immunoreactive cells, which seem to have no correspondence in other tetraconates. Furthermore, we show that not all immunoreactive neurons produce serotonin, but have the capability for serotonin uptake. Comparisons with the patterns of serotonin‐containing neurons in major tetraconate taxa suggest a close phylogenetic relationship of Remipedia, Cephalocarida, and Hexapoda, supporting the Miracrustacea hypothesis. J. Comp. Neurol., 2016. © 2016 Wiley Periodicals, Inc. J. Comp. Neurol. 525:79–115, 2017. © 2016 Wiley Periodicals, Inc.
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影响因子：
3.4
作者：
Stemme T;Iliffe TM;Bicker G;Harzsch S;Koenemann S
通讯作者：
Koenemann S
影响因子：
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DOI：
--
发表时间：
2007
期刊：
The Journal of comparative neurology
影响因子：
--
作者：
M. Stern;S. Knipp;Gerd Bicker
通讯作者：
Gerd Bicker
DOI：
10.1073/pnas.0306212101
发表时间：
2004-03-16
影响因子：
11.1
作者：
Fanenbruck, M;Harzsch, S;Wägele, JW
通讯作者：
Wägele, JW
影响因子：
2.5
作者：
Pech, Ulrike;Pooryasin, Atefeh;Fiala, Andre
通讯作者：
Fiala, Andre