Principles of bone marrow processing and progenitor cell/mononuclear cell concentrate collection in a continuous flow blood cell separation system.
Principles of bone marrow processing and progenitor cell/mononuclear cell concentrate collection in a continuous flow blood cell separation system.
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连续流动血细胞分离系统中骨髓处理和祖细胞/单核细胞浓缩物收集的原理。
DOI：
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发表时间：
1995
期刊：
影响因子：
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通讯作者：
A. Deisseroth
中科院分区：
文献类型：
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作者：
J. Hester;G. Rondon;Y. Huh;M. J. Lauppe;R. Champlin;A. Deisseroth
应用连续流单采技术处理骨髓以收集单核祖细胞群似乎遵循与从外周血收集单核细胞相同的原理。然而，与外周血不同，在外周血中，在手术过程中从血管外部位动员细胞对最终细胞产量有显著贡献，当合并原始骨髓时，可用于从骨髓中回收的祖细胞的全部量存在于原始骨髓中。然后，该过程变成尝试最佳回收感兴趣的细胞同时排除污染的红细胞和髓系细胞的过程。本研究报告了使用连续流动血细胞分离器从自体和同种异体移植的收获骨髓中回收MNC、CD 33+、CD 34+和CD 34 +/DR-细胞的方案的开发、影响目标细胞回收的变量以及对输注处理细胞的临床反应。
The application of continuous flow apheresis technology to processing bone marrow for collection of the mononuclear progenitor cell population appears to follow the same principles as collection of mononuclear cells from peripheral blood. Unlike peripheral blood, however, where mobilization of cells from extravascular sites during the procedures contributes significantly to the final cell yield, the entire quantity of progenitor cells available for recovery from marrow is present in the original marrow when it is pooled. The process then becomes one of attempting optimal recovery of the cells of interest while excluding contaminating erythrocytes and cells of the myeloid series. This study reports the development of a protocol for recovery of MNC, CD33+, CD34+, and CD34+/DR- cells from harvested marrow for autologous and allogeneic transplants using a continuous flow blood cell separator, the variables influencing the recovery of the cells of interest and the clinical response to infusion of the processed cells.
影响因子：
2.9
作者：
Lasky,LC;VanBuren,N;Weisdorf,DJ;Filipovich,A;McGlave,P;Kersey,JH;McCullough,J;Ramsay,NK;Blazar,BR
通讯作者：
Blazar,BR