Successful allogeneic cryopreserved marrow transplantation.

Successful allogeneic cryopreserved marrow transplantation.
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成功同种异体冷冻骨髓移植。

DOI:
10.1046/j.1537-2995.1989.29289146840.x
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发表时间:
1989
期刊:
影响因子:
2.9
通讯作者:
Blazar,BR
Blazar,BR
中科院分区:
医学3区
文献类型:
--
作者:
Lasky,LC;VanBuren,N;Weisdorf,DJ;Filipovich,A;McGlave,P;Kersey,JH;McCullough,J;Ramsay,NK;Blazar,BR

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冷冻保存已广泛应用于自体骨髓移植(BMT),但在异基因BMT中的应用有限。我们在这里描述6例成功的移植后异基因骨髓移植与冷冻保存的骨髓。患者患有Wiscott-Aldrich综合征、骨硬化症、再生障碍性贫血、急性淋巴细胞性白血病、急性非淋巴细胞性白血病和慢性髓细胞性白血病,年龄范围为5个月至35岁。在一例病例中,T细胞被移除以预防移植物抗宿主病。由于各种原因,骨髓被冷冻。将血沉棕黄层细胞在10%DMSO中以受控速率冷冻,并在液氮中储存6至49 d。移植后13 ~ 37 d移植物植入(WBC > 1000/uL × 3 d)。在4/4例数据可用的病例中,记录了植入物的供体来源,1例为细胞遗传学,2例为红细胞分型,4例为限制性片段长度多态性。3例患者在BMT后21、21和42个月存活且良好。这些结果表明,冷冻骨髓可以成功地用于异基因骨髓移植
Cryopreservation has been used extensively in autologous marrow transplantation (BMT), but there has been limited use in allogeneic BMT. We describe here 6 cases of successful engraftment following allogeneic BMT with cryopreserved marrow. Patients suffered from Wiscott‐Aldrich syndrome, osteopetrosis, aplastic anemia, and acute lymphocytic, acute non‐lymphocytic, and chronic myelogenous leukemia, and ranged in age from 5 mos to 35 yrs. Marrow was collected using standard techniques. In one case T‐cells were removed to prevent graft‐vs‐host disease. Marrow was frozen for a variety of reasons. Buffy coat cells were frozen at controlled rate in 10% DMSO, and stored in liquid nitrogen for 6 to 49 d. Engraftment (WBC > 1000/uL × 3 d) occurred from 13 to 37 d post BMT. In 4 of 4 cases in which data are available, donor origin of engraftment was documented, 1 with cytogenetics, 2 with red cell typing, and 4 with restriction fragment length polymorphisms. 3 patients are alive and well 21, 21, and 42 months post BMT. These results suggest frozen marrow can be successfully used for allogeneic BMT
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