A Novel Whole Yeast-Based Subunit Oral Vaccine Against Eimeria tenella in Chickens.
A Novel Whole Yeast-Based Subunit Oral Vaccine Against Eimeria tenella in Chickens.
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DOI：
10.3389/fimmu.2022.809711
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发表时间：
2022
影响因子：
7.3
通讯作者：
Blake DP
中科院分区：
文献类型：
--
作者：
Soutter F;Werling D;Nolan M;Küster T;Attree E;Marugán-Hernández V;Kim S;Tomley FM;Blake DP
需要廉价、易于生产的口服疫苗来控制鸡球虫病，以减少该疾病对福利和经济表现的影响。在四项独立的研究中，开发了表达三种柔嫩艾美耳球虫抗原的酿酒酵母，并将其作为热灭活、冻干全酵母口服疫苗递送给鸡。疫苗接种后，海兰褐蛋鸡中低剂量攻击（250 个卵囊）后柔嫩艾美球虫复制减少（p<0.01）。同样，在病原水平攻击后，使用表达 EtAMA1、EtIMP1 和 EtMIC3 的酿酒酵母混合物进行疫苗接种的海兰褐蛋鸡的盲肠病变评分也有所降低（4,000 个柔嫩球虫卵囊；p<0.01）。与模拟接种的对照相比，接种过 15,000 个柔嫩球虫卵囊后，接种过的 Cobb500 肉鸡的平均体重增加显着增加（p<0.01）。因此，灭活重组酵母疫苗为控制球虫病提供了具有成本效益且可扩展的机会，与低收入和中等收入国家（LMIC）的肉鸡生产和鸡饲养相关。
Cheap, easy-to-produce oral vaccines are needed for control of coccidiosis in chickens to reduce the impact of this disease on welfare and economic performance. Saccharomyces cerevisiae yeast expressing three Eimeria tenella antigens were developed and delivered as heat-killed, freeze-dried whole yeast oral vaccines to chickens in four separate studies. After vaccination, E. tenella replication was reduced following low dose challenge (250 oocysts) in Hy-Line Brown layer chickens (p<0.01). Similarly, caecal lesion score was reduced in Hy-Line Brown layer chickens vaccinated using a mixture of S. cerevisiae expressing EtAMA1, EtIMP1 and EtMIC3 following pathogenic-level challenge (4,000 E. tenella oocysts; p<0.01). Mean body weight gain post-challenge with 15,000 E. tenella oocysts was significantly increased in vaccinated Cobb500 broiler chickens compared to mock-vaccinated controls (p<0.01). Thus, inactivated recombinant yeast vaccines offer cost-effective and scalable opportunities for control of coccidiosis, with relevance to broiler production and chickens reared in low-and middle-income countries (LMICs).
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DOI：
10.1186/s12711-018-0433-7
发表时间：
2018-11-21
期刊：
Genetics, selection, evolution : GSE
影响因子：
--
作者：
Boulton K;Nolan MJ;Wu Z;Psifidi A;Riggio V;Harman K;Bishop SC;Kaiser P;Abrahamsen MS;Hawken R;Watson KA;Tomley FM;Blake DP;Hume DA
通讯作者：
Hume DA
影响因子：
6.7
作者：
Lai L;Bumstead J;Liu Y;Garnett J;Campanero-Rhodes MA;Blake DP;Palma AS;Chai W;Ferguson DJ;Simpson P;Feizi T;Tomley FM;Matthews S
通讯作者：
Matthews S
影响因子：
6.7
作者：
Blake DP;Billington KJ;Copestake SL;Oakes RD;Quail MA;Wan KL;Shirley MW;Smith AL
通讯作者：
Smith AL
影响因子：
2.6
作者：
CHAPMAN, HD
通讯作者：
CHAPMAN, HD
影响因子：
3.3
作者：
Li, YP;Bang, DD;Zhang, MF
通讯作者：
Zhang, MF