A rapid, inexpensive and disposable point-of-care blood test for sickle cell disease using novel, highly specific monoclonal antibodies.
A rapid, inexpensive and disposable point-of-care blood test for sickle cell disease using novel, highly specific monoclonal antibodies.
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使用新型、高度特异性的单克隆抗体进行快速、廉价且一次性的镰状细胞病即时血液检测。
DOI：
10.1111/bjh.14298
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发表时间：
2016-11
影响因子：
6.5
通讯作者：
Geisberg M
中科院分区：
文献类型：
--
作者：
Quinn CT;Paniagua MC;DiNello RK;Panchal A;Geisberg M
Sickle cell disease (SCD) is a significant healthcare burden worldwide, but most affected individuals reside in low-resource areas where access to diagnostic testing may be limited. We developed and validated a rapid, inexpensive, disposable diagnostic test, the HemoTypeSC™, based on novel monoclonal antibodies (MAbs) that differentiate normal adult haemoglobin (Hb A), sickle haemoglobin (Hb S) and haemoglobin C (Hb C). In competitive enzyme-linked immunosorbent assays, each MAb bound only its target with <0.1% cross-reactivity. With the HemoTypeSC™ test procedure, the sensitivity for each variant was <5.0 g/l. The accuracy of HemoTypeSC™ was evaluated on 100 whole blood samples from individuals with common relevant haemoglobin phenotypes, including normal (Hb AA, N=20), carrier or trait (Hb AS, N=22; Hb AC, N=20), SCD (Hb SS, N=22; Hb SC, N=13), and Hb C disease (Hb CC, N=3). The correct haemoglobin phenotype was identified in 100% of these samples. The accuracy of the test was not affected by Hb F (0-94.8% of total Hb) or Hb A2 (0-5.6% of total Hb). HemoTypeSC™ requires <1 μl of whole blood and no instruments or power sources. The total time-to-result is <20 min. HemoTypeSC™ may be a practical solution for point-of-care testing for SCD and carrier status in low-resource settings.
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影响因子：
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作者：
Lervolino LG;Baldin PE;Picado SM;Calil KB;Viel AA;Campos LA
通讯作者：
Campos LA
影响因子：
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通讯作者：
Shevkoplyas, Sergey S.
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通讯作者：
STANKER, LH
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Shevkoplyas SS
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通讯作者：
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