Heparin-binding EGF-like growth factor promotes intestinal anastomotic healing.
Heparin-binding EGF-like growth factor promotes intestinal anastomotic healing.
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DOI：
10.1016/j.jss.2010.06.036
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发表时间：
2011-12
期刊：
影响因子：
--
通讯作者：
Besner GE
中科院分区：
文献类型：
--
作者：
Radulescu A;Zhang HY;Chen CL;Chen Y;Zhou Y;Yu X;Otabor I;Olson JK;Besner GE
我们已经积累了多条证据，支持HB-EGF保护肠道免受损伤的能力，并促进皮肤部分烧伤的愈合。本研究旨在探讨肝素结合的表皮生长因子样生长因子(HB-EGF)在肠吻合口愈合中的作用。HB-EGF(−/−)基因敲除(KO)小鼠(n=42)及其野生型(WT)小鼠(n=33)，以及HB-EGF转基因(TG)小鼠(n=26)及其(WT)小鼠(n=27)接受了回肠末端的分离和吻合。此外，WT小鼠(n=21)接受肠内注射HB-EGF(800µg/kg)，接受相同的手术程序。分别于术后第3天和第6天测定吻合口破裂压。组织切片苏木精-伊红染色评价吻合口愈合情况，印迹红染色评价胶原沉积情况。用抗血管性血友病因子抗体进行免疫组织化学染色观察血管生成情况。还记录了并发症和死亡率。与WT小鼠相比，HB-EGF KO小鼠术后爆裂压显著降低，愈合评分低，死亡率高，并发症发生率高。与WT小鼠相比，KO小鼠的胶原沉积和血管生成明显减少。相反，与WT小鼠相比，HB-EGF TG小鼠术后吻合口破裂压升高，愈合评分高，死亡率低，并发症发生率低，胶原沉积增加，血管生成增加。接受HB-EGF治疗的WT小鼠与未接受HB-EGF治疗的小鼠相比，爆裂压力增加。结果表明，HB-EGF在肠吻合口愈合过程中起重要作用。
We have accumulated multiple lines of evidence supporting the ability of HB-EGF to protect the intestines from injury and to augment the healing of partial-thickness scald burns of the skin. The aim of the current study was to investigate the role of heparin-binding EGF-like growth factor (HB-EGF) in intestinal anastomotic wound healing. HB-EGF(−/−) knockout (KO) mice (n= 42) and their HB-EGF(+/+) wild type (WT) counterparts (n= 33), as well as HB-EGF transgenic (TG) mice (n=26) and their (WT) counterparts (n=27), underwent division and reanastomosis of the terminal ileum. In addition, WT mice (n=21) that received enteral HB-EGF (800µg/kg) underwent the same operative procedure. Anastomotic bursting pressure was measured at 3 and 6 days postoperatively. Tissue sections were stained with hematoxylin & eosin to assess anastomotic healing, and Picrosirus red to assess collagen deposition. Immunohistochemistry using anti-von Willebrand factor antibodies was performed to assess angiogenesis. Complications and mortality were also recorded. HB-EGF KO mice had significantly lower bursting pressures, lower healing scores, higher mortality and higher complication rates postoperatively compared to WT mice. Collagen deposition and angiogenesis were significantly decreased in KO mice compared to WT mice. Conversely, HB-EGF TG mice had increased anastomotic bursting pressure, higher healing scores, lower mortality, lower complication rates, increased collagen deposition and increased angiogenesis postoperatively compared to WT mice. WT mice that received HB-EGF had increased bursting pressures compared to non-HB-EGF treated mice. Our results demonstrate that HB-EGF is an important factor involved in the healing of intestinal anastomoses.
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DOI：
10.1097/00004630-199803000-00002
发表时间：
1998-03-01
期刊：
JOURNAL OF BURN CARE & REHABILITATION
影响因子：
--
作者：
Cribbs, RK;Luquette, MH;Besner, GE
通讯作者：
Besner, GE
影响因子：
15.9
作者：
HOMMA, T;SAKAI, M;HARRIS, RC
通讯作者：
HARRIS, RC
影响因子：
9.3
作者：
Junttila, TT;Sundvall, M;Elenius, K
通讯作者：
Elenius, K
DOI：
10.1091/mbc.1.11.811
发表时间：
1990-10-01
期刊：
CELL REGULATION
影响因子：
--
作者：
BESNER, G;HIGASHIYAMA, S;KLAGSBRUN, M
通讯作者：
KLAGSBRUN, M
影响因子：
2.5
作者：
Guven, Aysel;Pehlivan, Mevlut;Cam, Meryem
通讯作者：
Cam, Meryem