Far-red tracer analysis of traumatic cerebrovascular permeability.
Far-red tracer analysis of traumatic cerebrovascular permeability.
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DOI：
10.1016/j.jss.2014.05.011
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发表时间：
2014-08
影响因子：
2.2
通讯作者：
Cox, Charles S., Jr.
中科院分区：
文献类型：
--
作者：
Liao, George P.;Olson, Scott D.;Kota, Daniel J.;Hetz, Robert A.;Smith, Philippa;Bedi, Supinder;Cox, Charles S., Jr.
血脑屏障（BBB）受损是继发性创伤性脑损伤的关键病理生理组成部分，其特征在于先前免疫豁免环境中的水肿和神经炎症。目前用于BBB渗透性的测定受到工作尺寸、苛刻的提取过程、通过吸光度的次优检测和宽激发荧光光谱的限制。在这项研究中，我们评估了Alexa Fluor 680（一种与葡聚糖生物结合的远红染料）作为提高分辨率和灵敏度的替代检测方法的可行性。在处死当天将Alexa Fluor静脉内引入三组：假手术组、受控皮质损伤（CCI）组和用已知可降低BBB渗透性的基于细胞的疗法治疗的CCI组。将大脑冠状切片，并使用红外激光扫描仪进行成像，以生成强度曲线图以及信号阈值图像，以区分具有不同程度渗透性的区域。线性曲线分析表明CCI在相应损伤深度的信号强度大于治疗组大鼠。阈值分析识别了低+窄阈值范围内的信号边缘。来自已知保留BBB的治疗组的积分信号显著低于单独CCI损伤的组。在高+宽信号强度阈值范围内无显著差异。Alexa Fluor 680红外光电检测和图像分析可以帮助检测创伤性脑损伤后BBB渗透性的不同程度，并且可能特别有助于证明BBB对治疗药物的风险区域的保护。
Blood brain barrier (BBB) compromise is a key pathophysiological component of secondary traumatic brain injury characterized by edema and neuroinflammation in a previously immune privileged environment. Current assays for BBB permeability are limited by working size, harsh extraction processes, suboptimal detection via absorbance, and wide excitation fluorescence spectra. In this study, we evaluate the feasibility of Alexa Fluor 680, a far-red dye bioconjugated to dextran, as an alternative assay to improve resolution and sensitivity. Alexa Fluor was introduced intravenously on day of sacrifice to three groups: Sham, Controlled Cortical Injury (CCI), and CCI treated with a cell based therapy known to reduce BBB permeability. The brains were sectioned coronally and imaged using an infrared laser scanner to generate intensity plot profiles as well as signal threshold images to distinguish regions with varying degrees of permeability. Linear plot profile analysis demonstrated greater signal intensity from CCI than treated rats at corresponding injury depths. Threshold analysis identified rims of signal at low + narrow threshold ranges. The integrated signals from a treatment group known to preserve the BBB were significantly less than the groups with CCI injury alone. There was no significant difference at high + wide signal intensity threshold ranges. Alexa Fluor 680 infrared photodetection and image analysis can aid in detecting differential degrees of BBB permeability following traumatic brain injury and may be particularly useful in demonstrating BBB preservation of at-risk regions in response to therapeutic agents.
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影响因子：
3.8
作者：
Harting, Matthew T.;Jimenez, Fernando;Adams, Sasha D.;Mercer, David W.;Cox, Charles S., Jr.
通讯作者：
Cox, Charles S., Jr.
影响因子：
3.5
作者：
Saw, Melanie M.;Chamberlain, Jenny;Ho, Kwok M.
通讯作者：
Ho, Kwok M.
影响因子：
9.3
作者：
Walker PA;Bedi SS;Shah SK;Jimenez F;Xue H;Hamilton JA;Smith P;Thomas CP;Mays RW;Pati S;Cox CS Jr
通讯作者：
Cox CS Jr
影响因子：
34.7
作者：
Neuwelt, Edward A.;Bauer, Bjoern;Fahlke, Christoph;Fricker, Gert;Iadecola, Constantino;Janigro, Damir;Leybaert, Luc;Molnar, Zoltan;O'Donnell, Martha E.;Povlishock, John T.;Saunders, Norman R.;Sharp, Frank;Stanimirovic, Danica;Watts, Ryan J.;Drewes, Lester R.
通讯作者：
Drewes, Lester R.
影响因子：
4.2
作者：
Wei, Xiao-Er;Zhang, Yu-Zhen;Li, Wen-Bin
通讯作者：
Li, Wen-Bin