Specific expression of lncRNA RP13-650J16.1 and TCONS_00023979 in prostate cancer
Specific expression of lncRNA RP13-650J16.1 and TCONS_00023979 in prostate cancer
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lncRNA RP13-650J16.1和TCONS 00023979在前列腺癌中的特异性表达
DOI：
10.1042/bsr20171571
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发表时间：
2018-10
影响因子：
4
通讯作者：
Wang Gongxian
中科院分区：
文献类型：
--
作者：
Zhou Xiaochen;Chen Qiong;Wang Haolin;Zhang Cheng;Fu Bin;Wang Gongxian
本研究旨在探讨两种长链非编码RNA（lncRNA）（RP13 - 650J16.1和TCONS_00023979）在前列腺癌（PCa）中的表达谱及其可能的调控机制。采用高通量基因芯片技术检测PCa及癌旁组织中lncRNA的表达谱。将RP13 - 650J16.1或TCONS_00023979的特异性siRNA转染到DU145细胞中。然后，通过定量实时PCR和Western印迹检测RP13 - 650J16.1、受体相关共激活因子3（RAC 3）、早幼粒细胞白血病（PML）和TCONS_00023979的相对表达。MTT法检测细胞增殖;用Transwell小室测定DU 145细胞的迁移能力。平板克隆形成实验检测细胞增殖能力和克隆形成能力。PCa组织中RP13 - 650J16.1和RAC 3表达上调，TCONS_00023979和PML表达下调。RP13 - 650J16.1的沉默可降低RAC 3的表达，TCONS_00023979的敲除也降低PML的表达。此外，用si-RP 13 - 650 J16.1转染后，DU 145细胞的增殖、迁移和集落形成能力降低，而用si-TCONS_00023979转染后，这些能力增加。总之，我们的研究结果表明，RP 13 - 650 J16.1可能是一个癌基因，TCONS_00023979可能是一个抑癌基因在PCa。
The aim of the present study was to explore the expression profile and the potential regulatory mechanism of two long non-coding RNAs (lncRNAs) (RP13-650J16.1 and TCONS_00023979) in prostate cancer (PCa). Expression profile of lncRNAs in PCa and paracancerous tissues were investgated by the high-throughput gene chip technology. Specific siRNA of RP13-650J16.1 or TCONS_00023979 was transfected into DU145 cells. Then, the relative expression of RP13-650J16.1, receptor-associated coactivator 3 (RAC3), promyelocytic leukemia (PML), and TCONS_00023979 was detected by quantitative real-time PCR and Western blotting. MTT assay was used to detect the proliferation of DU145 cells. The migration ability of DU145 cells was measured by Transwell chambers. Single cell proliferation and clonogenic ability were detected by plate clone formation assay. RP13-650J16.1 and RAC3 expression was up-regulated, and TCONS_00023979 and PML expression was down-regulated in PCa tissues. Silencing RP13-650J16.1 could decrease RAC3 expression, and knockout of TCONS_00023979 also reduced PML expression. Moreover, the ability of proliferation, migration, and colony formation of DU145 cells was decreased after transfected with si-RP13-650J16.1, while these abilities were increased after transfected with si-TCONS_00023979. Collectively, our findings demonstrated that RP13-650J16.1 might be an oncogene and TCONS_00023979 might be an antioncogene in PCa.
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DOI：
10.1891/9780826121646.0002
发表时间：
2018-09
期刊：
Cancer Rehabilitation
影响因子：
--
作者：
K. Miller;R. Siegel;R. Khan;A. Jemal
通讯作者：
K. Miller;R. Siegel;R. Khan;A. Jemal
影响因子：
30.8
作者：
Prensner, John R.;Iyer, Matthew K.;Sahu, Anirban;Asangani, Irfan A.;Cao, Qi;Patel, Lalit;Vergara, Ismael A.;Davicioni, Elai;Erho, Nicholas;Ghadessi, Mercedeh;Jenkins, Robert B.;Triche, Timothy J.;Malik, Rohit;Bedenis, Rachel;McGregor, Natalie;Ma, Teng;Chen, Wei;Han, Sumin;Jing, Xiaojun;Cao, Xuhong;Wang, Xiaoju;Chandler, Benjamin;Yan, Wei;Siddiqui, Javed;Kunju, Lakshmi P.;Dhanasekaran, Saravana M.;Pienta, Kenneth J.;Feng, Felix Y.;Chinnaiyan, Arul M.
通讯作者：
Chinnaiyan, Arul M.
影响因子：
11.2
作者：
Zhou, HJ;Yan, J;Tsai, NJ
通讯作者：
Tsai, NJ
影响因子：
4.3
作者：
G. Franck
通讯作者：
G. Franck
影响因子：
5.4
作者：
Zhu, Miaojun;Chen, Qin;Yu, Jianxiu
通讯作者：
Yu, Jianxiu