Mesenteric lymph drainage alleviates hemorrhagic shock-induced spleen injury and inflammation
Mesenteric lymph drainage alleviates hemorrhagic shock-induced spleen injury and inflammation
复制标题
肠系膜淋巴引流减轻失血性休克引起的脾损伤和炎症
DOI：
10.1590/s0102-865020190090000003
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发表时间：
2019-11
影响因子：
1.1
通讯作者：
Zhao Zi-gang
中科院分区：
文献类型：
--
作者：
Zhang Hong;Zhai Jia-yi;Du Hui-bo;Zhang Li-min;Li Lin-feng;Bian An-qi;Jiang Li-na;Zhao Zi-gang
摘要目的：探讨肠系膜淋巴引流对失血性休克小鼠脾损伤及脾组织炎性细胞因子表达的影响。方法：雄性C57小鼠随机分为假休克组、休克组和休克+引流组。休克组和休克+引流组均造成股动脉出血，维持平均动脉压（MAP）40±2 mmHg 90 min，复苏。休克+引流组复苏时行肠系膜淋巴引流。复苏3h后取脾组织进行组织学观察和细胞因子蛋白及mRNA表达分析。结果如下：与假手术组相比，休克组脾脏MyD88、TRAF6 mRNA表达及TIPE2、MyD88、TRIF、TRAF3蛋白表达均明显增加。休克+引流组脾脏病理损伤明显减轻，TIPE2、MyD88、TRIF、TRAF3和TRAF6的mRNA和蛋白表达均明显低于休克组。结论：结果提示，失血性休克后肠系膜淋巴引流可减轻失血性休克所致的脾损伤及炎性细胞因子的表达。
Abstract Purpose: To investigate the effect of mesenteric lymph drainage on the spleen injury and the expressions of inflammatory cytokines in splenic tissue in mice following hemorrhagic shock. Methods: Male C57 mice were randomly divided into the sham shock, shock and shock+drainage groups. The mice in both shock and shock+drainage groups suffered femoral artery bleeding, maintained mean arterial pressure (MAP) of 40±2 mmHg for 90 min, and were resuscitated. And mesenteric lymph drainage was performed in the shock+drainage group at the time of resuscitation. After three hours of resuscitation, the splenic tissues were harvested for the histological observation and protein and mRNA expression analysis of cytokines. Results: The spleen in the shock group revealed a significantly structural damage and increased mRNA expressions of MyD88 and TRAF6 and protein expressions of TIPE2, MyD88, TRIF and TRAF3 compared to the sham group. By contrast, the splenic pathological injury in the shock+drainage group was alleviated significantly, and the mRNA and protein expressions of TIPE2, MyD88, TRIF, TRAF3 and TRAF6 were significantly lower than those in the shock group. Conclusion: These results indicate that post-hemorrhagic shock mesenteric lymph drainage alleviates hemorrhagic shock-induced spleen injury and the expressions of inflammatory cytokines.
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DOI：
10.1016/j.surge.2012.03.003
发表时间：
2012-12
影响因子：
2.5
作者：
Deitch, Edwin A.
通讯作者：
Deitch, Edwin A.
DOI：
10.1016/j.bbadis.2017.08.024
发表时间：
2017-10
期刊：
Biochimica et biophysica acta. Molecular basis of disease
影响因子：
--
作者：
Warren M;Subramani K;Schwartz R;Raju R
通讯作者：
Raju R
DOI：
10.1097/00000542-196611000-00068
发表时间：
2020-02
期刊：
Clinical Management of Shock - The Science and Art of Physiological Restoration
影响因子：
--
作者：
Fevzi Sarper Türker
通讯作者：
Fevzi Sarper Türker
影响因子：
3.3
作者：
J. Sambol;Marlon A. Lee;F. Caputo;Kentaro Kawai;C. Badami;T. Kawai;E. Deitch;A. Yatani
通讯作者：
J. Sambol;Marlon A. Lee;F. Caputo;Kentaro Kawai;C. Badami;T. Kawai;E. Deitch;A. Yatani
DOI：
10.1111/j.1749-6632.2010.05713.x
发表时间：
2010-01-01
期刊：
LYMPHATICS IN THE DIGESTIVE SYSTEM: PHYSIOLOGY, HEALTH, AND DISEASE
影响因子：
--
作者：
Deitch, Edwin A.
通讯作者：
Deitch, Edwin A.