Immunosuppressive effects of red blood cells on monocytes are related to both storage time and storage solution.
Immunosuppressive effects of red blood cells on monocytes are related to both storage time and storage solution.
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DOI：
10.1111/j.1537-2995.2011.03348.x
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发表时间：
2012-04
期刊：
影响因子：
2.9
通讯作者：
Hall M
中科院分区：
文献类型：
--
作者：
Muszynski J;Nateri J;Nicol K;Greathouse K;Hanson L;Hall M
单核细胞功能降低与危重病的不良结局相关。认为PRBC损害单核细胞功能，但PRBC储存溶液与单核细胞抑制之间的关系尚不清楚。本研究旨在验证PRBC对单核细胞的免疫抑制作用与保存时间和保存液有关的假设。将来自健康成人供体的单核细胞与PRBC共培养，PRBC已在AS-1、AS-3或仅CPD中储存7、14或21天。然后用LPS刺激细胞，并测定其上清液的TNFα和IL-10。进行Transwell实验以评估细胞与细胞接触的作用。通过RT-PCR定量单核细胞mRNA表达。与对照组相比，所有组的LPS诱导的TNFα产生能力均降低，但仅CPD的PRBC比储存在AS-1（p = 0.007）和AS-3（p = 0.006）中的PRBC更抑制单核细胞功能。IL-10的产生在所有组中均得到保持或增加。较长的储存时间与AS-1和AS-3组TNFα产生能力的降低有关，但与CPD无关。防止细胞与细胞接触并不能消除PRBC对单核细胞反应性的抑制作用。PRBC暴露与LPS诱导的TNFA mRNA表达降低相关（所有组p < 0.05）。仅CPD的PRBC比用添加剂溶液储存的PRBC更抑制单核细胞功能。即使在没有细胞间接触的情况下，TNFα的产生也会减少，并且在mRNA水平上受损。需要进一步的工作来了解防腐剂溶液在这一过程中的作用。
Reduced monocyte function is associated with adverse outcomes from critical illness. PRBC are thought to impair monocyte function but relationships between PRBC storage solution and monocyte suppression are unknown. This study was designed to test the hypothesis that immunosuppressive effects of PRBC on monocytes are related to both storage time and preservative solution. Monocytes from healthy adult donors were co-cultured with PRBC that had been stored in AS-1, AS-3, or CPD-only for 7, 14, or 21 days. Cells were then stimulated with LPS and their supernatants assayed for TNFα and IL-10. Transwell experiments were performed to evaluate the role of cell-to-cell contact. Monocyte mRNA expression was quantified by RT-PCR. LPS-induced TNFα production capacity was reduced compared to controls for all groups, but CPD-only PRBC suppressed monocyte function more than PRBC stored in AS-1 (p = 0.007) and AS-3 (p = 0.006). IL-10 production was preserved or augmented in all groups. Longer storage time was associated with reduced TNFα production capacity for AS-1 and AS-3 groups but not CPD. Preventing cell-to-cell contact did not eliminate the inhibitory effect of PRBC on monocyte responsiveness. PRBC exposure was associated with decreased LPS-induced TNFA mRNA expression (p < 0.05 for all groups). CPD-only PRBC suppressed monocyte function more than PRBC stored with additive solutions. TNFα production was reduced even in the absence of cell-to-cell contact and was impaired at the mRNA level. Further work is needed to understand the role of preservative solutions in this process.
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DOI：
10.1164/rccm.200711-1637oc
发表时间：
2008-07-01
影响因子：
24.7
作者：
Bateman, Scot T.;Lacroix, Jacques;Randolph, Adrienne G.
通讯作者：
Randolph, Adrienne G.
影响因子：
2.9
作者：
Gauvin, France;Spinella, Philip C.;Tucci, Marisa
通讯作者：
Tucci, Marisa
影响因子：
2.9
作者：
Karam, Oliver;Tucci, Marisa;Le Deist, Francoise
通讯作者：
Le Deist, Francoise
DOI：
10.1073/pnas.0504271103
发表时间：
2006-01-03
影响因子：
11.1
作者：
Gavrilin, MA;Bouakl, IJ;Wewers, MD
通讯作者：
Wewers, MD
影响因子：
9.6
作者：
Doughty, L;Carcillo, JA;Janosky, J
通讯作者：
Janosky, J