Cerebral Organoids as an Experimental Platform for Human Neurogenomics.
Cerebral Organoids as an Experimental Platform for Human Neurogenomics.
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DOI：
10.3390/cells11182803
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发表时间：
2022-09-08
期刊：
影响因子：
6
通讯作者：
Salama, Sofie R.
中科院分区：
文献类型：
--
作者：
Nowakowski, Tomasz J.;Salama, Sofie R.
关键词：
大脑皮层根据一系列可遗传的神经发育指令在发育早期形成。尽管大脑皮层及其基本的六层结构在进化过程中保持着高度的保守性，但在哺乳动物中可以发现皮层大小和折叠的显著差异，包括人类前额叶皮层的不成比例的扩张。然而，我们对神经发育过程的机械理解绝大多数来自啮齿动物模型，这些模型未能捕捉到许多人类丰富的皮层发育特征。随着多能干细胞和体外分化神经组织三维培养物的技术的出现，脑类器官已经成为一个实验平台，概括了人类大脑发育的几个标志。在这篇综述中，我们讨论了大脑类器官作为发育中的人脑的实验模型的优点和局限性。我们强调了技术开发中的创新，这些创新旨在提高其对体内大脑发育的保真度，并讨论了最近使用脑类器官研究再生和大脑进化以及开发神经和神经精神疾病模型的努力。
The cerebral cortex forms early in development according to a series of heritable neurodevelopmental instructions. Despite deep evolutionary conservation of the cerebral cortex and its foundational six-layered architecture, significant variations in cortical size and folding can be found across mammals, including a disproportionate expansion of the prefrontal cortex in humans. Yet our mechanistic understanding of neurodevelopmental processes is derived overwhelmingly from rodent models, which fail to capture many human-enriched features of cortical development. With the advent of pluripotent stem cells and technologies for differentiating three-dimensional cultures of neural tissue in vitro, cerebral organoids have emerged as an experimental platform that recapitulates several hallmarks of human brain development. In this review, we discuss the merits and limitations of cerebral organoids as experimental models of the developing human brain. We highlight innovations in technology development that seek to increase its fidelity to brain development in vivo and discuss recent efforts to use cerebral organoids to study regeneration and brain evolution as well as to develop neurological and neuropsychiatric disease models.
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影响因子：
23.9
作者：
Bershteyn M;Nowakowski TJ;Pollen AA;Di Lullo E;Nene A;Wynshaw-Boris A;Kriegstein AR
通讯作者：
Kriegstein AR
影响因子：
64.5
作者：
Andersen J;Revah O;Miura Y;Thom N;Amin ND;Kelley KW;Singh M;Chen X;Thete MV;Walczak EM;Vogel H;Fan HC;Paşca SP
通讯作者：
Paşca SP
影响因子：
56.9
作者：
Bonni, A;Sun, Y;Greenberg, ME
通讯作者：
Greenberg, ME
DOI：
10.16607/j.cnki.1674-6708.2025.16.038
发表时间：
2025-08-25
期刊：
科技传播
影响因子：
--
作者：
周婧
通讯作者：
--
影响因子：
64.8
作者：
Bhaduri A;Sandoval-Espinosa C;Otero-Garcia M;Oh I;Yin R;Eze UC;Nowakowski TJ;Kriegstein AR
通讯作者：
Kriegstein AR