Production of serpins using baculovirus expression systems.
Production of serpins using baculovirus expression systems.
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使用杆状病毒表达系统生产丝氨酸蛋白酶抑制剂。
DOI：
10.1016/s1046-2023(03)00209-3
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Clayman,GaryL
中科院分区：
文献类型：
--
作者：
Jayakumar,Arumugam;Cataltepe,Sule;Kang,Ya'an;Frederick,MitchellJ;Mitsudo,Kenji;Henderson,Ying;Crawford,SueE;Silverman,GaryA;Clayman,GaryL
Headpin is a novel serine proteinase inhibitor (serpin) that is downregulated in many established HNSCC tumor cell lines and human oral SCC specimens. The use of the bacterial and yeast expression systems for headpin resulted in poor yields and proteins with low inhibitory activity. To circumvent these problems, we have developed a baculovirus–insect cell system for high-yield expression as well as fully functional protein. Here, we describe the strategies and methods used to express headpin in an insect cell heterologous system. In addition, procedures to purify the recombinant proteins are described. A metal affinity column followed by a gel-filtration chromatography provides a rapid and efficient method for large quantity preparation of headpin. This method should be useful as an alternative expression system for those serpins that are not purifiable when expressed using the Escherichia coli or yeast expression system.
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DOI：
10.1109/t-su.1984.31522
发表时间：
1984-01-01
期刊：
IEEE TRANSACTIONS ON SONICS AND ULTRASONICS
影响因子：
--
作者：
FELLINGHAM, LL;SOMMER, FG
通讯作者：
SOMMER, FG
肝脏和脾脏霍奇金病的超声检查。
影响因子：
2.6
作者：
T. Sekiya;S. Meller;D. Cosgrove;V. McCready
通讯作者：
V. McCready
肝硬化：具有窄带滤波的美国图像。
影响因子：
19.7
作者：
Sommer,G;Stern,R;Chen,HS
通讯作者：
Chen,HS
随机频域组织表征：在“体内”人类脾脏中的应用。
影响因子：
4.2
作者：
F. Sommer;L. Joynt;D. L. Hayes;A. Macovski
通讯作者：
A. Macovski
恶性淋巴瘤的磁共振成像、胸部放射线摄影、计算机断层扫描和超声检查
影响因子：
1.3
作者：
R. Nyman;S. Rehn;B. Glimelius;H. Hagberg;A. Hemmingsson;P. Lindgren;A. Magnusson
通讯作者：
A. Magnusson