Competitive protein adsorption on polysaccharide and hyaluronate modified surfaces.
Competitive protein adsorption on polysaccharide and hyaluronate modified surfaces.
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DOI：
10.1080/08927014.2011.585711
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发表时间：
2011-05
期刊：
影响因子：
2.7
通讯作者：
Eckmann DM
中科院分区：
文献类型：
--
作者：
Ombelli M;Costello L;Postle C;Anantharaman V;Meng QC;Composto RJ;Eckmann DM
我们测量了牛血清白蛋白（BSA）和纤维蛋白原（Fg）在六种不同裸露的、葡聚糖和透明质酸修饰的硅表面上的吸附，这些硅表面由两种葡聚糖接枝密度和三种透明质酸（HA）钠盐制成，这些透明质酸钠盐分别来自人脐带、鸡冠和动物流行链球菌。膜的厚度和表面形貌取决于HA的分子量和浓度。BSA:Fg混合物的竞争性吸附增强了BSA在表面的覆盖。右旋糖酐化根据氧化态差异减少了蛋白质在表面上的吸附。透明质化被证明对蛋白质覆盖具有最大的抵抗力，相当于最具抵抗力的右旋化表面。对蛋白质吸附的抗性与所用透明质酸的类型无关。当每个物种的体积蛋白浓度从20µg ml−1变化到40µg ml−1时，Fg在硅上的覆盖率增加了4倍，而BSA和Fg在葡聚糖和HA上的吸附对蛋白质体积浓度的依赖性要小得多。
We measured adsorption of bovine serum albumin (BSA) and fibrinogen (Fg) onto six distinct bare and dextran- and hyaluronate-modified silicon surfaces created using two dextran grafting densities and three hyaluronic acid (HA) sodium salts derived from human umbilical cord, rooster comb and streptococcus zooepidemicus. Film thickness and surface morphology depended on HA molecular weight and concentration. BSA coverage was enhanced on surfaces upon competitive adsorption of BSA:Fg mixtures. Dextranization differentially reduced protein adsorption onto surfaces based on oxidation state. Hyaluronization was demonstrated to provide the greatest resistance to protein coverage, equivalent to that of the most resistant dextranized surface. Resistance to protein adsorption was independent of the type of hyaluronic acid utilized. With changing bulk protein concentration from 20 to 40 µg ml−1 for each species, Fg coverage on silicon increased by 4×, whereas both BSA and Fg adsorption on dextran and HA were far less dependent of protein bulk concentration.
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