Naive stem cell blastocyst model captures human embryo lineage segregation.
Naive stem cell blastocyst model captures human embryo lineage segregation.
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DOI：
10.1016/j.stem.2021.04.031
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发表时间：
2021-06-03
期刊：
影响因子：
23.9
通讯作者：
Guo G
中科院分区：
文献类型：
--
作者：
Yanagida A;Spindlow D;Nichols J;Dattani A;Smith A;Guo G
人类幼稚多能细胞可分化为胚外滋养外胚层和下胚层。在这里，我们描述了一个人类胚胎模型（类胚）产生的自组织。滋养外胚层的短暂诱导导致在3天内形成胚泡样结构。类胚细胞由三个组织层组成，显示出独特的谱系标记，模仿天然胚泡。单细胞转录组分析证实滋养外胚层、下胚层和上胚层的分离与人类胚胎高度一致。这个多功能和可扩展的系统为人类胚胎研究提供了一个强大的实验模型。产生结构和组成与胚胎相似的人类类胚细胞滋养外胚层、下胚层和幼稚上胚层谱系的分离胚胎的转录组保真度简单、高效和可扩展的程序早期胚胎的可靠干细胞模型将为研究人类发育和不育开辟新的途径。Yanagida及其同事使用幼稚干细胞产生人类胚泡样结构，包括胚外膜和胚胎的三个基础组织层。
Human naive pluripotent cells can differentiate into extraembryonic trophectoderm and hypoblast. Here we describe a human embryo model (blastoid) generated by self-organization. Brief induction of trophectoderm leads to formation of blastocyst-like structures within 3 days. Blastoids are composed of three tissue layers displaying exclusive lineage markers, mimicking the natural blastocyst. Single-cell transcriptome analyses confirm segregation of trophectoderm, hypoblast, and epiblast with high fidelity to the human embryo. This versatile and scalable system provides a robust experimental model for human embryo research. Generation of human blastoids with structure and composition similar to the embryo Segregation of trophectoderm, hypoblast, and naive epiblast lineages Transcriptome fidelity with the embryo Simple, efficient, and scalable procedure Reliable stem cell models of the early embryo would open new approaches to studying human development and infertility. Yanagida and colleagues used naive stem cells to generate human blastocyst-like structures comprising the three founding tissue layers for the extraembryonic membranes and the embryo.
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影响因子：
21.3
作者：
Shahbazi, Marta N.;Jedrusik, Agnieszka;Vuoristo, Sanna;Recher, Gaelle;Hupalowska, Anna;Bolton, Virginia;Fogarty, Norah M. E.;Campbell, Alison;Devito, Liani G.;Ilic, Dusko;Khalaf, Yakoub;Niakan, Kathy K.;Fishel, Simon;Zernicka-Goetz, Magdalena
通讯作者：
Zernicka-Goetz, Magdalena
DOI：
10.1242/dev.167833
发表时间：
2018-11-09
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Boroviak T;Stirparo GG;Dietmann S;Hernando-Herraez I;Mohammed H;Reik W;Smith A;Sasaki E;Nichols J;Bertone P
通讯作者：
Bertone P
影响因子：
5.9
作者：
Guo G;von Meyenn F;Santos F;Chen Y;Reik W;Bertone P;Smith A;Nichols J
通讯作者：
Nichols J
影响因子：
64.5
作者：
Petropoulos S;Edsgärd D;Reinius B;Deng Q;Panula SP;Codeluppi S;Plaza Reyes A;Linnarsson S;Sandberg R;Lanner F
通讯作者：
Lanner F
影响因子：
64.5
作者：
Takashima Y;Guo G;Loos R;Nichols J;Ficz G;Krueger F;Oxley D;Santos F;Clarke J;Mansfield W;Reik W;Bertone P;Smith A
通讯作者：
Smith A