Single cell transcriptome analysis of human, marmoset and mouse embryos reveals common and divergent features of preimplantation development.
Single cell transcriptome analysis of human, marmoset and mouse embryos reveals common and divergent features of preimplantation development.
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DOI：
10.1242/dev.167833
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发表时间：
2018-11-09
期刊：
影响因子：
--
通讯作者：
Bertone P
中科院分区：
文献类型：
--
作者：
Boroviak T;Stirparo GG;Dietmann S;Hernando-Herraez I;Mohammed H;Reik W;Smith A;Sasaki E;Nichols J;Bertone P
小鼠胚胎是哺乳动物着床前发育的典型模型。单细胞分析的最新进展允许详细分析其他真兽目物种（包括人类）的胚胎发生，以区分啮齿动物范例中保守的不同调控程序和信号通路。在这里，我们通过单细胞RNA-seq鉴定并比较了人类、绒猴和小鼠胚胎的转录特征。合子基因组激活与所有三个物种中多梳抑制复合物的存在相关，而核糖体生物合成作为灵长类动物胚胎的主要属性出现，支持母体沉积RNA的长期翻译。我们发现转座因子的表达特征是物种、阶段和谱系特异性的。灵长类动物上胚层中的多能性网络缺乏在小鼠中起作用的某些调节因子，但包括与滋养层特化相关的WNT组分和基因。原始内胚层同一性的GATA 6、SOX 17和GATA 4标志物的顺序激活在灵长类动物中是保守的。出乎意料的是，OTX 2也与人和非人灵长类动物胚泡中的原始内胚层特化相关。我们的跨物种分析划分了保守和灵长类动物特有的植入前发育特征，并强调了早期哺乳动物胚胎发生的分子适应性。 对来自三种哺乳动物的阶段匹配的单细胞基因表达数据的分析揭示了早期胚胎发生和谱系特化的保守和灵长类特异性调节。
The mouse embryo is the canonical model for mammalian preimplantation development. Recent advances in single cell profiling allow detailed analysis of embryogenesis in other eutherian species, including human, to distinguish conserved from divergent regulatory programs and signalling pathways in the rodent paradigm. Here, we identify and compare transcriptional features of human, marmoset and mouse embryos by single cell RNA-seq. Zygotic genome activation correlates with the presence of polycomb repressive complexes in all three species, while ribosome biogenesis emerges as a predominant attribute in primate embryos, supporting prolonged translation of maternally deposited RNAs. We find that transposable element expression signatures are species, stage and lineage specific. The pluripotency network in the primate epiblast lacks certain regulators that are operative in mouse, but encompasses WNT components and genes associated with trophoblast specification. Sequential activation of GATA6, SOX17 and GATA4 markers of primitive endoderm identity is conserved in primates. Unexpectedly, OTX2 is also associated with primitive endoderm specification in human and non-human primate blastocysts. Our cross-species analysis demarcates both conserved and primate-specific features of preimplantation development, and underscores the molecular adaptability of early mammalian embryogenesis. Analysis of stage-matched, single-cell gene expression data from three mammalian species reveals conserved and primate-specific regulation of early embryogenesis and lineage specification.
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DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
10.5
作者：
de Lau W;Peng WC;Gros P;Clevers H
通讯作者：
Clevers H
影响因子：
48
作者：
Engstrom, Par G.;Steijger, Tamara;Sipos, Botond;Grant, Gregory R.;Kahles, Andre;Raetsch, Gunnar;Goldman, Nick;Hubbard, Tim J.;Harrow, Jennifer;Guigo, Roderic;Bertone, Paul
通讯作者：
Bertone, Paul
影响因子：
64.8
作者：
BRAUDE, P;BOLTON, V;MOORE, S
通讯作者：
MOORE, S
影响因子：
4.6
作者：
Artus, Jerome;Panthier, Jean-Jacques;Hadjantonakis, Anna-Katerina
通讯作者：
Hadjantonakis, Anna-Katerina