Changes in external pH rapidly alter plant gene expression and modulate auxin and elicitor responses.
Changes in external pH rapidly alter plant gene expression and modulate auxin and elicitor responses.
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DOI：
10.1111/j.1365-3040.2010.02161.x
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发表时间：
2010-09
期刊：
影响因子：
--
通讯作者：
Rasmusson AG
中科院分区：
文献类型：
--
作者：
Lager I;Andréasson O;Dunbar TL;Andreasson E;Escobar MA;Rasmusson AG
pH值对根来说是一个高度可变的环境因子，植物细胞可以通过改变外胞体pH值来获取营养并响应细胞外信号。然而，令人惊讶的是，外界pH值对植物基因表达的影响很少。我们使用微阵列来研究外部pH值是否会影响全局基因表达。在拟南芥根系中，881个基因在培养基pH从6.0转变为4.5后8小时的转录丰度发生了至少2倍的变化，这表明pH是全球基因表达的主要影响因素。一些基因在20分钟内响应，并且在幼苗培养的叶片中也观察到基因响应。从生长和转录反应来评估，pH为4.5的处理与非生物胁迫无关。然而，观察到的全球基因表达模式表明，对pH、生长素和病原体激发子的反应之间存在冗余和相互作用。此外，基因表达的主要变化与细胞壁修饰和Ca2+信号传导有关。相应地，在ph响应基因的启动子中观察到Ca2+/钙调素相关基序的显着过度表达。这强烈表明植物的pH值识别涉及细胞内Ca2+。总的来说，这些结果强调了以前被低估的pH值在植物对环境的反应中的作用。
pH is a highly variable environmental factor for the root, and plant cells can modify apoplastic pH for nutrient acquisition and in response to extracellular signals. Nevertheless, surprisingly few effects of external pH on plant gene expression have been reported. We have used microarrays to investigate whether external pH affects global gene expression. In Arabidopsis thaliana roots, 881 genes displayed at least 2-fold changes in transcript abundance 8 h after shifting medium pH from 6.0 to 4.5, identifying pH as a major affector of global gene expression. Several genes responded within 20 min, and gene responses were also observed in leaves of seedling cultures. The pH 4.5 treatment was not associated with abiotic stress, as evaluated from growth and transcriptional response. However, the observed patterns of global gene expression indicated redundancies and interactions between the responses to pH, auxin and pathogen elicitors. In addition, major shifts in gene expression were associated with cell wall modifications and Ca2+ signaling. Correspondingly, a marked overrepresentation of Ca2+/calmodulin-associated motifs was observed in the promoters of pH-responsive genes. This strongly suggests that plant pH recognition involves intracellular Ca2+. Overall, the results emphasize the previously underappreciated role of pH in plant responses to the environment.
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DOI：
10.1085/jgp.99.4.615
发表时间：
1992-04
期刊：
The Journal of general physiology
影响因子：
--
作者：
Blatt MR
通讯作者：
Blatt MR
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
5.3
作者：
Geisler DA;Johansson FI;Svensson AS;Rasmusson AG
通讯作者：
Rasmusson AG
DOI：
10.1007/bf00211406
发表时间：
1995-10-01
期刊：
EUROPEAN BIOPHYSICS JOURNAL
影响因子：
--
作者：
HEDRICH, R;MORAN, O;PALME, K
通讯作者：
PALME, K
影响因子：
7.3
作者：
Blossfeld, Stephan;Gansert, Dirk
通讯作者：
Gansert, Dirk